Author of

• 20149

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  MA 20 - Recent Advances in Pulmonology/Endoscopy (ID 685)

  • Event: WCLC 2017
  • Type: Mini Oral
  • Track: Pulmonology/Endoscopy
  • Presentations: 1
  • Moderators:C. Lee, S. Sasada
  • Coordinates: 10/18/2017, 14:30 - 16:15, F205 + F206 (Annex Hall)

  • 24384

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    MA 20.06 - Discerning Lung Cancer Cell Patterns with Confocal Endomicroscopy (ID 8640)

    15:05 - 15:10  |  Author(s): R.M. Ortiz
    • Abstract
    • Presentation
    • Slides

    Background:
    Probe-based confocal endomicroscopy (pCLE) allows confocal microscopy of lung tissue in vivo but limited evidence is available. The objective was to discriminate pCLE patterns of lung cancer in vivo.
    
    Method:
    Fluorescence properties of methylene blue (MB) were examined ex vivo in confocal microscope. Next, 15 regions of the central airways were studied in vivo with pCLE and a representative image chosen for analysis with ImageJ software. Biopsy was performed for final diagnosis.
    
    Result:
    Ex vivo study showed no differences between 1% and 2% MB concentrations and rapid extinction of fluorescence after 10 minutes of MB application (figure). In vivo study included samples of bronchial mucosa (n = 6), inflammation (n = 3) and tumor (n = 6). pCLE image evaluation (table) showed inflammation and tumor nuclei were bigger (except SCLC) and occupied a greater area. Fluorescence of tumor nuclei was more intense. Non fluorescent area was inferior for both inflammation and tumor samples. Number of nuclei could not discriminate between normal and tumor.Figure 1 Table. Imaging features evaluated in pCLE frames

    	Area occupied by nuclei (µm[2])	Intensity of nuclei (UA)	Mean size of nuclei (µm[2])	Non-fluorescent area (µm[2])	Number of nuclei (µm[2])
    Bronchial epithelium (mean(SD))	97,769(9,451)	126(9)	107(10)	67,100(12,567)	937(84)
    Inflammation (mean(SD))	117,381(22,166)	122(27)	127(15)	35,124(32,630)	933(225)
    B cell lymphoma	138,354	145	185	49,269	746
    Adenocarcinoma	155,033	198	177	5,225	875
    Squamous cell carcinoma	102,805	145	155	54,301	663
    Small cell lung cancer	107,201	157	63	11,257	1,687
    Non-small cell lung cancer	113,173	187	122	32,359	926
    Hamartoma	120,188	145	114	25,438	1,058

    
    
    
    
    Conclusion:
    1. MB fluorescence is unaffected by stain concentration 2. There is exponential extinction of MB over time 3. Lung cancer cell pattern distinction in vivo is feasible Funded by Fundació MaratóTV3, SEPAR and FUCAP
    
    

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