Author of

• 20195

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  P3.16 - Surgery (ID 732)

  • Event: WCLC 2017
  • Type: Poster Session with Presenters Present
  • Track: Surgery
  • Presentations: 1
  • Moderators:
  • Coordinates: 10/18/2017, 09:30 - 16:00, Exhibit Hall (Hall B + C)

  • 24238

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    P3.16-015 - Rapid Identification of Micropapillary or Solid Component for Early-Stage Lung Adenocarcinoma (ID 8304)

    09:30 - 09:30  |  Author(s): M.J. Underwood
    • Abstract
    • Slides

    Background:
    Sublobar resection may be less than ideal for lung adenocarcinoma (ADC) with even minor micropapillary (MIP) or solid (SOL) component, given that they carry a higher incidence of locoregional recurrence. Rapid identification of these subtypes would help decision-making on the extent of resection for early-stage ADC.
    
    Method:
    Antibody arrays of adhesion and apoptosis molecules were applied for 8-pair ADCs with (≥5%) or without (<5%) MIP/SOL component to identify the differentially expressed proteins, which were further validated by Western blot. A semi-dry dot-blot (SDB) system that visualizes the presence of the target proteins, modifying from the dot-blot method, was used to for diagnosing MIP/SOL existence in a prospective cohort of 45 clinical stage I ADCs that received operation. Resected specimens were reviewed according to the new IASLC/ATS/ERS classification and each component was recorded in 5% increments.
    
    Result:
    Insulin-like growth factor-binding protein 2 (IGFBP2) and P-cadherin was found more frequently in the MIP/SOL positive group and thereby setting as the target proteins in the SDB system for detection. A total of 46 nodules with a mean size of 2.4±0.8 cm was enrolled, including 10 (21.7%) with MIP and 16 (34.8%) with SOL component. The specificity and sensitivity for detecting MIP/SOL existence through SDB method were 72.0% and 90.5%, respectively. The average test duration was 25.6±1.9 minutes. Interestingly, the test successfully diagnosed one of the two synchronic lesions to have SOL component (Figure 1).
    
    Conclusion:
    Detecting IGFBP2 and P-cadherin via SDB method may have a potential role in the rapid identification of MIP/SOL components in early-stage lung ADC. Figure 1. (A) Semi-dry dot-blot system identified the expression of IGFBP2 and P-cadherin by the comparison of chromogen density to reference. The positive result indicated the existence of micropapillary or solid components. (B) Synchronous lesions with or without solid component showed different test result. Figure 1
    
    
    
    

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