Author of

• 20186

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  P3.07 - Immunology and Immunotherapy (ID 723)

  • Event: WCLC 2017
  • Type: Poster Session with Presenters Present
  • Track: Immunology and Immunotherapy
  • Presentations: 1
  • Moderators:
  • Coordinates: 10/18/2017, 09:30 - 16:00, Exhibit Hall (Hall B + C)

  • 24117

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    P3.07-008 - Development of Immunomonitoring Assays for Dendritic Cell-Based Lung Cancer Immunotherapy (ID 9804)

    09:30 - 09:30  |  Author(s): I. Vančurová
    • Abstract
    • Slides

    Background:
    Allogeneic cancer cell lines might serve as a universal source of tumor antigens in the development of dendritic cell-based cancer vaccines. We showed that selected antigenic profile of lung cancer cell lines overlaps with antigenic profile of primary non/small cell lung cancer (NSCLC) tumors. However, it is unclear if T cells responses to all of these antigens can be detected in blood of NSCLC patients.
    
    Method:
    Peripheral blood mononuclear cells (PBMCs) of NSCL patients were stimulated and re-stimulated by commercially available mixes of antigenic peptides derived from these antigens over the course of 10 days. Tumor antigen-specific CD8[+] and CD4[+] T cells were characterized by IFN-γ production, granzyme B, perforin, an d CD137 or CD154 expression by flow cytometry. In addition, the expression of inhibitory molecules TIM3, CTLA-4, PD-1 and LAG-3 were evaluated on CD8[+] and CD4[+] T cells from PBMC of NSCLC patients. We further analysed 6 populations of myeloid-derived suppressor cells (MDCS) by a multicolor flow cytometry and their possible functional suppression by qPCR analysis of ARG1 and iNOS expression in PBMC and downregulation of CD3zξ in T cells from patient’s PBMCs compared to T cells from PBMCs of healthy donors.
    
    Result:
    We were able to detect tumor antigen-specific IFN-γ, Granzyme B and Perforin producing CD8[+] and CD4[+] T cells as well as expression of inhibitory molecules TIM3, CTLA-4, PD-1 and LAG-3. We were also able to detect populations of MDSCs in blood of NSCLC patients as well as healthy donors.
    
    Conclusion:
    This data will allow us to develop a protocol for immunomonitoring studies of the effectivity of dendritic cell-based lung cancer immunotherapy in ongoing phase I lung cancer clinical trial (NCT02470468).
    
    

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