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X. Zhu



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    P3.05 - Early Stage NSCLC (ID 721)

    • Event: WCLC 2017
    • Type: Poster Session with Presenters Present
    • Track: Early Stage NSCLC
    • Presentations: 1
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      P3.05-009 - Hsa_circ_0044013: A Potential Novel Diagnostic Biomarker of Lung Adenocarcinoma (ID 9488)

      09:30 - 09:30  |  Author(s): X. Zhu

      • Abstract
      • Slides

      Background:
      Circular RNAs (circRNAs) are a special class of endogenous RNAs exhibiting stable structure and high tissue- and developmental-specific expression. Recent studies have found that aberrant expression of circular RNAs plays important roles in carcinogenesis and tumor progression. However, their value in the diagnosis of lung adenocarcinoma remains unknown. In this study, we focused on hsa_circ_0044013, which was found to be upregulated in lung adenocarcinoma tissues in our previous microarray analysis. The purpose of this study was to clarify the possible role of hsa_circ_0044013 and to define its diagnostic value in lung adenocarcinoma.

      Method:
      Expression levels of hsa_circ_0044013 in 30 paired lung adenocarcinoma tissues and adjacent non-tumor tissues were measured by real-time quantitative reverse transcription polymerase chain reaction（qRT-PCR）. We also measured expression levels of hsa_circ_0044013 in three NSCLC cell lines by qRT-PCR. Then, we detected expression levels of hsa_circ_0044013 in the plasma samples from 70 lung adenocarcinoma patients and 70 healthy individuals. Differences in expression levels of hsa_circ_0044013 were analyzed using the paired t-test. Then, the association between the expression level of hsa_circ_0044013 and the clinicopathological features of patients with lung adenocarcinoma was further analyzed. A receiver operating characteristic (ROC) curve was generated to evaluate the diagnostic value.

      Result:
      Hsa_circ_0044013 was significantly upregulated in lung adenocarcinoma tissues compared with adjacent non-tumor tissues (P = 0.0143). Its expression levels in two detected lung adenocarcinoma cell lines (A549, SPCA1) were upregulated than those in HBE and H1703. Moreover, Hsa_circ_0044013 was detected to be upregulated in plasma samples from lung adenocarcinoma patients (P = 0.0046). Its expression levels were significantly correlated with tumor diameter and TNM stage. The areas under the ROC curve (AUC) of hsa_circ_0044013 in plasma were up to 0.753. The sensitivity and specificity of the combination were 0.592 and 0.959.

      Conclusion:
      These results suggested that hsa_circ_0044013 may be a novel non-invasive biomarker for the diagnosis of lung adenocarcinoma.

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