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R. Sagi-Eisenberg
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P3.02 - Biology/Pathology (ID 620)
- Event: WCLC 2017
- Type: Poster Session with Presenters Present
- Track: Biology/Pathology
- Presentations: 1
- Moderators:
- Coordinates: 10/18/2017, 09:30 - 16:00, Exhibit Hall (Hall B + C)
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P3.02-096 - The Interaction Between Mast Cells and Lung Cancer Cells Through Extracellular Vesicles (ID 10200)
09:30 - 09:30 | Author(s): R. Sagi-Eisenberg
- Abstract
Background:
Exosomes are nano-sized extracellular vesicles that mediate cell-to-cell communication by transferring molecules. Mast cells are secretory cells that complete their differentiation and maturation in the micro-environment of tissues containing blood vessels, and were observed in the periphery of certain tumors. It has been hypothesized that tumor cells affect their environment by passing genetic codes such as miRNA through exosomes. In this study, we investigated the presence of exosomal content in the micro-environment of lung cancer cells as a communication mechanism that drives tumor development.
Method:
Human mast cells (HMC1) were exposed to Human lung cancer cells (H1299) in in vitro setting. Exosomes were isolated from mast cells alone, lung cancer cells alone and from a co-culture of mast cells and lung cancer cells. As a control, exosomes were produced from mast cells that were activated by membranes of lung cancer cells. Exosomes quantity and quality were analyzed including its miRNA composition. Samples were run on an HTG EdgeSeq Processor using the HTG EdgeSeq miRNA WT assay. Each assay contains 2102 probes for miRNA, including 13 housekeeper genes, 5 negative process controls, and 1 positive process control. The HTG EdgeSeq Parser was used to align the FASTQ files to the probe list to collate the data. CPM (counts per million) was normalized and differential expression was analyzed using DESeq2.
Result:
We exposed mast cells to membranes of lung cancer cells, in order to examine induction of mast cells. Out of 2066 miRNA analyzed only 3 were significantly upregulated: miR-31-5p, miR-100-5p and miR-125b-5p. Interestingly, the profile of the pathways activated by these 3 upregulated miRNAs shows the focal adhesion and adherens junctions as one of the top results. A comparison was made between miRNA expression of exosomes from mast cells vs. exosomes from lung cancer cells. Out of 2066 miRNA analyzed, a total of 112 miRNA were differentely expressed. 79 miRNAs were downregulated, for example hsa-miR-6802-5p downregulated in HMC1 in comparison with H1299, and moreover hsa-miR-4700-5p. 33 miRNAs were significantly upregulated, for example miR-146b-5p/miR-146a-5p. Top upregulated miRNAs were tested using KEGG pathway and predicted to lead to the activation of the pathways like viral carcinogenesis, pathways in cancer and cell cycle.
Conclusion:
Mast cells and cancer cells do have common and discriminating exosomal content. Interestingly, mast cells have been induced by the presence of lung cancer cells’ membranes, through a change in the canonical pathways of focal adhesion, by the upregulation of only 3 miRNAs.
