Author of

• 18974

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  P3.02 - Biology/Pathology (ID 620)

  • Event: WCLC 2017
  • Type: Poster Session with Presenters Present
  • Track: Biology/Pathology
  • Presentations: 1
  • Moderators:
  • Coordinates: 10/18/2017, 09:30 - 16:00, Exhibit Hall (Hall B + C)

  • 24020

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    P3.02-072 - MiR-33b Inhibits Lung Adenocarcinoma Cell Epithelial-Mesenchymal Transition Through CeRNA Regulatory Network (ID 9402)

    09:30 - 09:30  |  Author(s): C. Hu
    • Abstract
    • Slides

    Background:
    Lung cancer is a signifcant health problem worldwide and the mechanism of lung cancer cell metastasis has not been fully elucidated.Epithelial-mesenchymal transition (EMT) is not only an important biological mechanism for natural physiological process but also has been proved to be related to cancer metastasis. Further studies have pointed out that EMT plays a indispensable role in the entire process of metastasis.In recent years , many miRNAs have been demonstrated to target EMT-related proteins, such as E-cadherin and Vimentin to regulate the initiation and progression of EMT. The preliminary study of my research group has indicated that miR-33b plays an inhibitory role in the EMT of lung cancer and two genes ，ZEB1 and Twist1 ，are downstream target of miR-33b.Then snail1 which is EMT-related proteins like ZEB1 has also been founded that a potential correlation with miR-33b.A ceRNA hypothesis tells that If different transcripts have the same miRNA binding site, it can combine with miRNA competely and influence its inhibitory effect on the target, and then adjust the expression of each other.Snial1、ZEB1 and Twist1 three genes would bind to the same miRNA which is miR-33b，but the relations among this three genes and miR-33b still need to be clarified
    
    Method:
    Western blotting、RT-pcr、Immunofluorescence 、Immunohistochemistry and Luciferase assay were used to explore the relations among Snial1、ZEB1、Twist1 and miR-33b.The effects of miR-33b and Snail1 on lung adenocarcinoma cancer cell functions were investigated by using migration and invasion assays.Construct gene expression vectors and transfect the cells to research the regulative relations among Snial1、ZEB1、Twist1 and miR-33b.Tumor formation in nude mice to certify the relations between Snail1 and miR-33b in vitro.
    
    Result:
    miR-33b suppresses EMT and lung adenocarcinoma cell proliferation and migration in vitro.miR-33b directly targets SNAIL1.miR-33b regulates lung adenocarcinoma cells by down-regulating Snial1 expression.miR-33b inhibits tumor growth through the SNAIL1 in vivo.Snial1、ZEB1、Twist1 influence each other.
    
    Conclusion:
    Snial1、ZEB1 and Twist1 fuction as cerna for each other.MiR-33b inhibits lung adenocarcinoma cell epithelial-mesenchymal transition through ceRNA regulatory network
    
    

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