Author of

• 18974

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  P3.02 - Biology/Pathology (ID 620)

  • Event: WCLC 2017
  • Type: Poster Session with Presenters Present
  • Track: Biology/Pathology
  • Presentations: 1
  • Moderators:
  • Coordinates: 10/18/2017, 09:30 - 16:00, Exhibit Hall (Hall B + C)

  • 24006

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    P3.02-058 - Detection of ROS1 Rearrangements in 508 Russian Patients with Non-Small Cell Lung Cancer (NSCLC) (ID 10252)

    09:30 - 09:30  |  Author(s): M. Gikalo
    • Abstract
    • Slides

    Background:
    Identification of specific oncogenic genetic drivers can improve survival in a subset of patients with NSCLC, eligible for modern targeted therapies. Detection of ROS1 rearrangements became standard of diagnostic, but some challenges are under discussion in this setting. We report the first results of large-scale testing in an all-Russian Network for Molecular Diagnostics in cancer patients supported by RUSSCO
    
    Method:
    Tumor samples from 508 Russian pts negative for EGFR mutations and ALK rearrangements were submitted for ROS1 testing from September 2016 to May 2017. Initial screening was performed using IHC assay exploiting D4D6 antibody (Cell Signaling Technologies) and OptiView DAB IHC detection kits with OptiView Amplification kit (Ventana Medical Systems). All cases scored as positive/equivocal by IHC were forwarded for FISH.
    
    Result:
    456 cases were negative by IHC, 12 cases (2%) were positive by the both assays and 31 cases were scored as equivocal (6,1%) with negative FISH results in 29. Nine cases (2%) failed because of serious defects of preanalytic managing or lack of tumor tissue. Three cases were additionally reviewed and scored as heterogenic positive by IHC and heterogenic by FISH with close-to-border amount of rearranged cells. One case harbored concomitant L858R EGFR mutation confirmed by repeated PCR investigation. All these cases are under additional investigation for revealing of possible role of ROS1 rearranged clone in oncogenesis.
    
    Conclusion:
    Identification of ROS1 rearrangements in NSCLC patients has some challenges possibly indicating more complicated role of this aberration in NSCLC.
    
    

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