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P. Choudhari
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P3.02 - Biology/Pathology (ID 620)
- Event: WCLC 2017
- Type: Poster Session with Presenters Present
- Track: Biology/Pathology
- Presentations: 1
- Moderators:
- Coordinates: 10/18/2017, 09:30 - 16:00, Exhibit Hall (Hall B + C)
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P3.02-036 - Feasibility Study to Evaluate Patterns of Metastases and Effect of Surgery on Lung Cancer Xenografts with Differing Sensitivity to EGFR TKI (ID 8660)
09:30 - 09:30 | Author(s): P. Choudhari
- Abstract
Background:
Lung cancer cell lines with differing sensitivities to the epidermal growth factor receptor tyrosine kinase inhibitor, Erlotinib have different invasive and metastatic potential. Surgical intervention may play a role in altering the pattern of metastases and survival. Studying these patterns may help in designing trials to evaluate the efficacy of peri-operative EGFR inhibition.
Method:
Two lung cancer cell lines with known different sensitivity to Erlotinib were selected; A549 known to be resistant to Erlotinib (sensitive to Everolimus) and HCC 827, known to be sensitive to Erlotinib. 12 NOD SCID mice were injected with A549 and 17 NOD SCID with HCC 827 as xenografts in the thigh. FDG-18 PET scans were performed in all mice thrice, at 7-10 days, 4 weeks and at 6 weeks. 8 of the 12 mice with A549 cell line and 12 of the 17 with HCC 827 cell line underwent surgery for local tumour at 4-5 weeks from inoculation. The rest of the mice; 4 in A549 group and 5 in HCC 827 group served as controls. All mice were subjected to autopsy at death.
Result:
For the mice with the HCC 827 cell line: Local invasive potential was 90%. 40% of the mice in the control group and 33.3% of the mice in the operated group produced metastases. Survival was similar in operated and control groups (126 versus 127 days). For the mice with A549 cell line: Local invasion and metastases were seen in all mice. The survival in the group undergoing surgery was 129 days versus 107 days in the control group.
Conclusion:
Both the cell lines have good invasive potential, A549 cell line scored over HCC827 in producing metastases. All the metastases were noted only in the lungs. This study can serve as a background to evaluate the role of peri-operative inhibition with epidermal growth factor receptor tyrosine kinase inhibitors and/or mTor inhibitors.
