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H. Gao
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P3.02 - Biology/Pathology (ID 620)
- Event: WCLC 2017
- Type: Poster Session with Presenters Present
- Track: Biology/Pathology
- Presentations: 1
- Moderators:
- Coordinates: 10/18/2017, 09:30 - 16:00, Exhibit Hall (Hall B + C)
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P3.02-017 - Apoptosis-Related Protein in Non Small Cell Lung Cancer: Correlation of Clinicopathologic, Molecular Characteristics and Prognosis (ID 10011)
09:30 - 09:30 | Author(s): H. Gao
- Abstract
Background:
The role of apoptosis protein that regulates the biological behavior of non-small-cell lung carcinoma (NSCLC) is still controversial. We aimed to investigate the expression of apoptosis-related protein (survivin and bcl-2 family) and to identify their association with the clinicopathologic parameters and prognosis of patients with NSCLC.
Method:
Immunohistochemical (IHC) staining of pro-apoptotic (bax, bad, bim), anti-apoptotic proteins (bcl-2, survivin) and proliferation marker (Ki-67) was performed on tissue microarray sections from tumor tissues of 373 NSCLCs. Correlations between the expression of the above proteins and clinicopathologic, molecular features (EGFR mutation, KRAS mutation, ALK translocation and MET amplification) and prognostic significance were analyzed.
Result:
Analysis of the two main subtypes of NSCLC, adenocarcinoma (ADC) and squamous cell carcinoma (SCC) individually showed that different markers were significant in the different subtypes. The expression of survivin, bax and Ki-67 was significantly different in ADC and SCC. In ADC, the increased expression of survivin was associated with the presence of vascular invasion, lymph node metastasis and tumor recurrences, but we did not find any correlation with survivin expression and clinicopathological parameters in SCC. The increased expression of bax was associated with the presence of lymphatic invasion and tumor recurrence in SCC, but no correlation with bax expression and clinicopathological parameters was observed in ADC. Kaplan–Meier survival analysis showed that survivin and Ki-67 were significant prognostic markers for ADC, whereas bax was a significant prognostic marker for SCC. Patients with high survivin and Ki-67 expression had significantly shorter disease-free survival as well as shorter overall survival than those with low survivin and Ki-67 expression. In SCC, patients with high bax expression had significantly shorter disease-free survival than those with low bax expression. Multivariate Cox analysis confirmed that survivin, Ki-67 and bax were independent prognostic factors in ADC and SCC, respectively. Among the six markers, only high bim expression was significantly related with the presence of MET amplification (p = 0.025), however, other five markers lack significant associations with EGFR, KRAS, ALK, MET gene status.
Conclusion:
Our results suggest that survivin and Ki-67 are independent negative prognostic factors in ADC and bax is an independent negative prognostic factor in SCC. Testing for these markers will help to determine the clinical relevance of NSCLC. Bim may possibly play a role in MET-amplified lung cancer.
