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S. Ma
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P3.02 - Biology/Pathology (ID 620)
- Event: WCLC 2017
- Type: Poster Session with Presenters Present
- Track: Biology/Pathology
- Presentations: 1
- Moderators:
- Coordinates: 10/18/2017, 09:30 - 16:00, Exhibit Hall (Hall B + C)
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P3.02-015 - 433MHz Microwave Radiation Induces G2/M Checkpoint Arrest and Promotes Apoptosis under Hyperthermia in Non-Small Cell Lung Cancer Cells (ID 8876)
09:30 - 09:30 | Author(s): S. Ma
- Abstract
Background:
Postoperative hyperthermia has been applied as an important adjuvant therapy to enhance the efficacy of traditional cancer treatment. To provide an update on the recent knowledge about the mechanisms of microwave (MW) hyperthermia on NSCLC cells using special MW device in vitro.
Method:
The various NSCLC cells (H460, PC-9 and H1975) were exposed to hyperthermia treatment using water bath or MW applicator. Cell survival was determined by CCK-8 assay. Cell apoptosis and cell cycle distributions were performed by Flow cytometry. Western blot assay was used to detect cell cycle arrest related molecular changes. Assessment of intracellular ROS changes in cells was performed using fluorescence probes DCFH-DA staining.
Result:
The MW hyperthermia can significantly inhibit cell growth in 3 cell lines, compared with water bath heating system. Flow cytometry results showed that the apoptotic cells increased significantly in MW hyperthermia treatment group, mean value of percent apoptosis treated by the MW hyperthermia was 12.45%, 40.00%, 37.70% in H460, PC-9, and H1975 cells respectively. The cell cycle distribution analysis showed that MW hyperthermia induced G2/M phase arrest. Western blot results suggested that MW thermal down-regulated the expressions of cyclinB1/cdc2, cdc 25c and p-cdc-25c, up-regulated expression of p21. Assessment of intracellular ROS change showed that MW hyperthermia induced production of ROS in NSCLC cells.
Conclusion:
Exposure of NSCLC cells to 433 MHz microwave hyperthermia induces ROS production and cell cycle arrest, which in turn induces cyclinB1/cdc 2 complex inactivation through inhibiting expressions of cdc 25c and increasing expression of p21, and finally promotes cell apoptosis. The heat treatment by water bath has not the same effect. This study suggests that microwave hyperthermia may be a potential therapeutic option for treating NSCLC in future.
