Author of

• 18974

  +

  P3.02 - Biology/Pathology (ID 620)

  • Event: WCLC 2017
  • Type: Poster Session with Presenters Present
  • Track: Biology/Pathology
  • Presentations: 1
  • Moderators:
  • Coordinates: 10/18/2017, 09:30 - 16:00, Exhibit Hall (Hall B + C)

  • 23957

    +

    P3.02-010 - Significant Increase of Blood Extracellular Vesicles in Pulmonary Vein as Potential Prognostic Biomarker for Lung Cancer Patients (ID 9973)

    09:30 - 09:30  |  Author(s): Y.B. Choy
    • Abstract

    Background:
    Exracellular vesicles(EVs) are endosome-derived nano-size (30-1000 nm) vesicles released from many cell types including cancer cells. Previous researches have demonstrated that the level of EVs is increased in cancer patients than healthy controls. This study was conducted to evaluate the variation of EVs-count in the proximal tumor-drainage vessel and peripheral vessels during surgery for VX2 rabbit lung cancer model and primary lung cancer patients
    
    Method:
    A total of 6 rabbits were used in this study (3 in normal group, 31 in lung cancer group). Rabbit VX2 lung cancer model was made by real-time computed tomographic guided inoculation of VX2 cancer. Two weeks after injection, blood was collected from rabbit peripheral vessel and pulmonary vein (proximal tumor-drainage vein). A total of 3 healthy controls and 31 patients with primary lung cancer who had pT2aN0 (stage IB) and underwent lobectomy were selected. For each patient, 3 ml of blood was sampled from the radial artery (peripheral vessel) before surgery and from pulmonary vein of the primary tumor site (proximal tumor-drainage vein) during surgical procedure. Healthy controls blood were collected from peripheral vessels. EVs were isolated by serial centrifugation followed by ExoQuick[TM] and quantitative analysis was performed by NanoSight and western blotting.
    
    Result:
    EVs-count was not different in normal rabbit model according to blood sampling sites (peripheral vessel: 2.78 x 10[8] particles/ml, pulmonary vessel: 2.64 x 10[8]; p = 0.104). However, in rabbit lung cancer model, EVs were increased by 623.5% in peripheral vessels (1.73 x 10[9 ]particles/ml; p = 0.003) and 787.9% in proximal tumor-drainage vein (2.08 x 10[9] particles/ml; p = 0.001) comparing to those of normal rabbits. Moreover, we confirmed that EVs-count in VX2 lung cancer model was increased by 120.0% (p = 0.05) on the proximal tumor-drainage vein than peripheral vessel. In human blood samples, peripheral blood derived EVs were increased by 181.6% in lung cancer patient in comparison with healthy controls (2.44 x 10[8] particles/ml in healthy control, 4.43 x 10[8] particles/ml in lung cancer patients; p = 0.04). And, EVs were significantly increased by 700.8% in pulmonary vein of the primary tumor site (1.71 x 10[9] particles/ml; p = 0.0001) comparing to peripheral vessels in lung cancer patients
    
    Conclusion:
    The increase of EVs was more prominent in tumor-drainage veins than peripheral vessels in animal cancer models and lung cancer patients. We suggest that increase of EVs from tumor-drainage veins may provide more relevant prognostic information of the lung cancer patients comparing to those from peripheral vessel after surgery.