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L. Li
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P2.02 - Biology/Pathology (ID 616)
- Event: WCLC 2017
- Type: Poster Session with Presenters Present
- Track: Biology/Pathology
- Presentations: 1
- Moderators:
- Coordinates: 10/17/2017, 09:30 - 16:00, Exhibit Hall (Hall B + C)
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P2.02-062 - The MicroRNAs Associated with Recurrence and Metastasis of Stage I Lung Adenocarcinoma (ID 9634)
09:30 - 09:30 | Author(s): L. Li
- Abstract
Background:
Up to 30% stage I non-small cell lung cancer (NSCLC) patients undergone the tumor resection suffer from recurrence within 5 years. Applicable prognostic biomarkers are required to identify those with high risk of relapse after the surgery, for modified adjuvant therapy to potentially improve survival. This study aims to identify the microRNAs (miRNAs) that associate with recurrence and metastasis of stage I lung adenocarcinoma (ADC).
Method:
Two groups of stage I lung ADC patients were enrolled, with 40 cases for each. For the patients in the group RM, the tumor recurrence and/or metastasis occurred within 2 years after the surgical resection. For the patients in the group NRM, the tumor recurrence and/or metastasis had not reappeared for 5 years after the surgery. The tumor tissues were enriched from formalin-fixed and paraffin-embedded tissue sections, and the total RNAs were extracted. The Agilent human microRNA microarrays were used to generate miRNA profiles and explore the aberrantly expressed miRNAs in the samples investigated. The interesting differentially expressed miRNAs between the two groups were then validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in an independent cohort of 80 cases of stage I lung ADC, which was also divided into two groups, the RM and NRM.
Result:
The miRNA expression profiling revealed that a panel of miRNAs were differentially expressed between the group RM and group NRM. Cluster analysis showed that this panel of miRNAs could distinguish patients in the group RM from those in the group NRM. Moreover, according to target gene prediction and KEGG pathway analysis of these miRNAs, predicted target genes of 2 miRNAs (miR-3621 and miR-2467-3p) in the panel are involved in the non-small cell lung cancer pathways. The expression of these miRNAs were tested subsequently by qRT-PCR in the tumor samples of the independent cohort, using miR-191 as an internal reference. The preliminary data showed that among them, the mean relative expression fold change (2[-ΔCt]) of miR-2467-3p in the group RM was significantly lower than that in the NRM (p = 0.014).
Conclusion:
Our finding suggests that a panel of miRNAs may be regarded as candidate biomarkers for prognosis of early stage lung ADC. Among them miR-2467-3p was aberrantly down-regulated in the tumor tissues of patients in the group RM; accordingly, miR-2467-3p (and its predicted target genes) may play a role in recurrence and/or metastasis of stage I lung ADC after the surgical resection.
