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S. Withers



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    P2.02 - Biology/Pathology (ID 616)

    • Event: WCLC 2017
    • Type: Poster Session with Presenters Present
    • Track: Biology/Pathology
    • Presentations: 1
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      P2.02-037 - CD14<Sup>+</Sup> Cell Tumor Microenvironment Infiltration Correlates with Poor Overall Survival in Patients with Early Stage Lung Cancer (ID 9322)

      09:30 - 09:30  |  Author(s): S. Withers

      • Abstract

      Background:
      Despite undergoing curative-intent therapy, patients with early stage non-small cell lung cancer (NSCLC) have reduced survival rates of 43-73% due to the risk of distant metastasis(1-3). This underscores the acute need to identify new biomarkers for improved prognostication and to better understand the underlying biology that drives poor outcomes.

      Method:
      Tumor tissue from 189 patients with NSCLC who underwent curative intent surgery was stained for CD14 by IHC and qualitatively scored for low, moderate, or high expression. CD14 expression groups for all patients and stage I patients alone were analyzed for overall survival. In vitro studies were performed utilizing a coculture system of human lung cancer cell lines and freshly isolated CD14[+ ]cells.

      Result:
      We found that the level of CD14[+ ]cells within the tumor microenvironment (TME) was strongly associated with overall survival in all patients (p<0.001) and stage I patients alone (p=0.006). Patients with high CD14 TME staining had a median overall survival of 5.5 years versus 8.3 years and 10.7 years for moderate or low CD14 staining, respectively. The intensity of CD14 TME infiltration was associated with an advanced stage at time of diagnosis (p=0.049) and more positive lymph nodes found at surgery (p=0.012). The potential advantages of TME CD14[+ ]cells were investigated through a coculture system. Tumor growth kinetics were not altered with coculture. Lung cancer cells cocultured with CD14[+ ]cells recovered more quickly after exposure to chemotherapy. This improved recovery was observed only after a 48 hour coculture of tumor cells and CD14[+ ]cells. Figure 1



      Conclusion:
      CD14[+] cells are a prognostic marker within the TME of patients with resected lung adenocarcinoma. Our data suggests crosstalk with the CD14[+] cell infiltration results in a tumor survival benefit that drives poor patient outcomes.