Author of

• 18971

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  P1.02 - Biology/Pathology (ID 614)

  • Event: WCLC 2017
  • Type: Poster Session with Presenters Present
  • Track: Biology/Pathology
  • Presentations: 1
  • Moderators:
  • Coordinates: 10/16/2017, 09:30 - 16:00, Exhibit Hall (Hall B + C)

  • 22537

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    P1.02-053 - A New Strategy of Adjuvant Chemotherapy for Lung Cancer Based on the Expression of Anti-Aging Gene Klotho (ID 10001)

    09:30 - 09:30  |  Author(s): T. Inoue
    • Abstract

    Background:
    Adjuvant chemotherapy of lung cancer including cisplatin is recommended for patients with lung cancer p-stage II-IIIA in case of complete resection. However, at the present, treatment outcomes are not necessarily satisfactory, and there is not effective chemotherapy resume for each patient individually. Establishing effective chemotherapy resume for each patients individually and practicing personalized medicine of lung cancer are expected for improving treatment outcomes after operation of p-stage II-IIIA patients. We reported that anti-aging gene Klotho expression was a prognostic factor for small cell lung cancer and LCNEC so far. In this study, we aimed at revealing possibility of the Klotho gene expression as prognostic factor and effect prediction factor of the chemotherapy, and establishing personalized medicine for adjuvant chemotherapy of lung cancer.
    
    Method:
    We introduced the Klotho-plasmid into lung cancer cell A549 and established Klotho overexpressing cell, A549/Klotho. We examined the sensitivity test for various anticancer agents including pemetrexed, CDDP, paclitaxel, gefitinib, etc, using A549 cells and A549/Klotho cells.
    
    Result:
    We performed sensitivity test by MTT assay. A549/Klotho cells were more sensitive seven times against pemetrexed compared to A549 cells (IC50; 0.1 micro M). A549/Klotho cells were a little sensitive against docetaxel. There is no difference of sensitivity between A549/Klotho cells and A549 cells against molecular target drugs such as afatinib, alectinib, ceritinib, gefitinib, and osimertinib. In A549/Klotho cells expression of N-cadherin was completely inhibited by Western blot analysis.
    
    Conclusion:
    From these result, we conclude that overexpression of Klotho may regulate the sensitivity against pemetrexed through the inhibition of N-cadherin. In the future, we may establish a new strategy of adjuvant chemotherapy for lung cancer based on the expression of anti-aging gene Klotho.