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M.M. García Falcone
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P1.02 - Biology/Pathology (ID 614)
- Event: WCLC 2017
- Type: Poster Session with Presenters Present
- Track: Biology/Pathology
- Presentations: 1
- Moderators:
- Coordinates: 10/16/2017, 09:30 - 16:00, Exhibit Hall (Hall B + C)
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P1.02-035 - Human Papillomavirus Infection in Lung Squamous Cell Carcinoma and Correlation to p16 INK4a Expression from an Argentine Population (ID 9561)
09:30 - 09:30 | Author(s): M.M. García Falcone
- Abstract
Background:
Lung cancer is the leading cause of cancer death worldwide. In 1979, Syrjänen suggested a role for human papillomavirus (HPV) infection in bronchial carcinoma. Many studies have found HPV on lung carcinoma, predominantly in squamous cell carcinoma (SCC). There seems to be a geographical factor determining prevalence rates. In Latin America, only 2 studies, altogether including 51 cases of lung SCC, examined this association. However, data from Argentina is lacking. The aim of this study is to asses the incidence of HPV infection in lung SCC of Argentinean population, and to correlate with p16[INK4a ]expression from an Argentine population.
Method:
The study was approved by CEMIC’s Ethics Committee. Informed consent was obtained. Materials consisted of formalin-fixed paraffin-embedded (FFPE) tissue from 29 surgically excised and 11 transbronchial biopsies of primary L-SCC evaluated between 2006-2016. HPV Genotyping: On 50μm-thick slides, tumor was microdissected and DNA was extracted (columns method). Wide-spectrum HPV DNA (L1-ORF) was amplified by PCR. Positive specimens were genotyped by PCR for types 16 and 18. Immunohistochemistry: All p16 staining’s were performed on VENTANA BenchMark GX using antibody CINtec® p16. Staining patterns were interpreted on a binary way (positive or negative). Only cases with diffusely intense cytoplasmic and/or nucleic staining on tumor cell (TC) were considered positive. Cases in which the normal bronchial epithelium resulted p16 positive but TC were negative, were also registered.
Result:
HPV was isolated in 10/40 cases (25%). The details of HPV infection and the clinicopathological data is depicted on table 1.Clinicopathological features of SCC
HPV positive (n=10) HPV negative (n=30) Gender Female 5 11 Male 5 19 Age <50 1 - 50-60 - 21 >60 9 9 Smoking Never-smoker - - Smoker - - Unknown 10 30 Tumor cell differentiation Well 1 - Moderate - 10 Poorly 9 20 Keratinizing Non-keratinizing 9 27 Keratinizing 1 3 p16 positive on tumor cellls Positive 3 2 Negative 7 28 p16 on bronchial epithelium Positive 3 2 Negative 7 28 HPV type HPV 16 3 - HPV 18 5 - Co-infection HPV 16 and 18 2 - Specimen type Transbronchial biopsy 3 8 Surgical excision 7 22
Conclusion:
We detected an HPV infection rate of 25%. HPV18 was the common genotype. On 7 cases, normal bronchial epithelium was both p16 and HPV positive, suggesting that adjacent tumor tissue may be HPV infected. p16 should not be used as a surrogate marker for HPV infection, since it is only positive on 60% of cases.
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P2.01 - Advanced NSCLC (ID 618)
- Event: WCLC 2017
- Type: Poster Session with Presenters Present
- Track: Advanced NSCLC
- Presentations: 1
- Moderators:
- Coordinates: 10/17/2017, 09:00 - 16:00, Exhibit Hall (Hall B + C)
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P2.01-007 - Molecular Characterization of Non-Small Cell Lung Cancers (NSCLC) in Young Patients from an Argentine Population (ID 7951)
09:00 - 09:00 | Author(s): M.M. García Falcone
- Abstract
Background:
NSCLC is the leading cause of cancer-related deaths in Argentina. NSCLC is rarely observed in young adults (aged 18-40 years) and presents distinctive molecular characteristics. This study analyzed the prevalence of oncogenic molecular alterations in tumor samples from young adults treated at our institution. Different molecular biology techniques were used and treatment outcomes were reported.
Method:
Retrospective observational study of FFPE tumor samples from individuals aged 18-40 years, presenting stage IV lung adenocarcinoma. ALK fusions were studied by IHC (clone-D5F3) and confirmed with FISH-Vysis. The areas selected for molecular studies were micro-dissected, and DNA/RNA were purified. EGFR mutations were studied by Sanger. If available, targeted NGS was done with Colon and Lung. Cancer Research Panel v2 (CLRP) for DNA analysis; and/or Oncomine™ Panel Focus Assay (OFA) for DNA/RNA analysis. Both panels were performed in an Ion 520 chip sequenced in the Ion S5 Next Generation Sequencing Systems. The sequences obtained were analyzed in the Ion Reporter™ Software 5.2.1. The OFA was informed by Ion Torrent™ Oncomine™ Knowledgebase Reporter.
Result:
Six patients were included, 5/6 tumors were lung adenocarcinoma and 1/6, poorly differentiated carcinoma. The male:female ratio was 2:1. Median age was 35y (range 32-37) and all subjects had stage IV disease. EGFR and ALK were tested in all patient's samples, and 4/6 had NGS analysis. Five samples (83%) harbored known targetable oncogenic drivers: EGFR sensitizing mutations occurred in 3/5, ALK translocation with KRAS co-mutation in 1/5, and HER2 exon 20 insertions in 1/5. Only one sample without NGS was negative for the studied oncogenes. Targeted therapies were administered to 4/5 patients. Figure 1
Conclusion:
Our series shows a high prevalence of known actionable oncogenic drivers in young patients with NSCLC tumor. In this population an extensive molecular profiling of tumors is required to improve the treatment strategy.
