Author of

• 18974

  +

  P3.02 - Biology/Pathology (ID 620)

  • Event: WCLC 2017
  • Type: Poster Session with Presenters Present
  • Track: Biology/Pathology
  • Presentations: 1
  • Moderators:
  • Coordinates: 10/18/2017, 09:30 - 16:00, Exhibit Hall (Hall B + C)

  • 24040

    +

    P3.02-092 - CD151-Integrin-C-Kit Axis Plays an Important Role in the Pathogenesis of Non-Small Cell Lung Cancer (ID 8780)

    09:30 - 09:30  |  Presenting Author(s): Zeyi Liu
    • Abstract

    Background:
    CD151, a master regulator of laminin-binding integrins (α3β1、α6β1 and α6β1), assembles these integrins into complexes called tetraspanin-enriched microdomains. Hence, CD151 is well positioned to modulate integrin-dependent cell spreading, migration, signaling, and adhesion strengthening. Now, it has been shown to be involved in tumour progression. The molecular mechanism of CD151 in cancer is based on its ability to organize distribution and function of interacting proteins, ie, laminin-binding integrins (α3β1、α6β1 and α6β1), receptors for growth factors (HGFR, EGFR). Considering the fact that CD151-integrin complex can regulate receptors for growth factors (HGFR, EGFR), we hypothesized that CD151-integrin complex involving in the pathogenesis and acquired resistance to EGFR-TKIs in lung cancer.
    
    Method:
    o determine the expression of CD151 in NSCLC cell lines and tissues, quantitative real-time PCR (qRT-PCR), Immunohistochemistry (IHC) and Western blot were performed. Cell Counting Kit-8 assay was applied to evaluate the cell proliferation, and propyliodide organism (PI) staining was used to detect the cell cycle. Meanwhile we observed the alteration of cell proliferation and cell cycle after transient transfection with sh-CD151 and CD151 over-expressed into lung cancer cell lines. The Human Soluble Receptor Array Kit Non-Hematopoietic Panelprotein-kinase (R&D Systems, ARY012) and human RTKs phosphorylation antibody array. (RayBiotech Inc, Array-AAH-PRTK-G1), was performed according to manufacturer guidelines.
    
    Result:
    Figure 1The expression of CD151 was significantly increased in NSCLC cell lines and tissues. After transfected sh-CD151 or CD151 over-expressed vector into lung cancer cell lines, they showed significant growth-suppressing or promoting effect We utilized a human soluble receptor array and a human RTKs phosphorylation antibody array to investigate whether these and/or other oncogenic signaling pathways were activated downstream of CD151-integrin complex in NSCLC cells. Our results showed that CD151-integrin complex could regulated the growth and metastasis in NSCLC through c-kit signaling.
    
    
    
    Conclusion:
    CD151-integrin-c-kit axis plays an important role in the pathogenesis of non-small cell lung cancer