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Qi Wang



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    P1.01 - Advanced NSCLC (ID 757)

    • Event: WCLC 2017
    • Type: Poster Session with Presenters Present
    • Track: Advanced NSCLC
    • Presentations: 1
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      P1.01-078a - The Construction and Clinical Application of an Integrated Microfluidic Device for CTCs Detection in Patients with NSCLC  (ID 8379)

      09:30 - 09:30  |  Presenting Author(s): Qi Wang

      • Abstract
      • Slides

      Abstract not provided

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    P1.03 - Chemotherapy/Targeted Therapy (ID 689)

    • Event: WCLC 2017
    • Type: Poster Session with Presenters Present
    • Track: Chemotherapy/Targeted Therapy
    • Presentations: 1
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      P1.03-051 - Development of a Novel Microfluidic Device for Studying the Chemotaxis Mechanism of Bacterial Cancer Targeting (ID 7996)

      09:30 - 09:30  |  Presenting Author(s): Qi Wang

      • Abstract

      Background:
      The increasing prevalence of cancer cases worldwide and shortcomings of existing treatment methods including chemotherapy, radiation therapy, surgery and transplantation call for the immediate development of novel cancer therapies. Due to the non-specificity of current therapies, recognition between cancer cells and normal cells is a challenging dilemma in cancer therapeutics. Bacterial-mediated cancer therapy is a novel alternative treatment currently under intensive study. However, the exact mechanism of tumor degradation in bacterial-mediated cancer therapy is not fully understood and remains a challenging question for cancer therapeutics.

      Method:
      To characterize the mechanism of bacterial chemotactic preference towards cancer cells, we developed a novel microfluidic device for in vitro applications. The device, when used as model for lung cancer, provides simultaneous three-dimensional co-culture of multiple cell lines in separate culturing chambers and establishes constant concentration gradients of biochemical compounds in a central channel by diffusion through micro-channels. The quantification of preferential accumulation of bacteria towards a particular cell type was determined against established chemotactic gradient. Bacterial taxis behavior towards cancer and normal cells was measured in the two-chamber system using the fluorescence intensity of green fluorescence protein (GFP)-encoding bacteria. Furthermore, secretome and bioinformatic analysis of the cancer cells determined significant factors in bacterial cancer targeting. Figure 1



      Result:
      Using the microfluidic platform, E. coli clearly illustrated the preference for lung cancer cells (NCI-H460) which was attributed to biochemical factors secreted by carcinoma cells. Furthermore, secretome and bioinformatic analysis of the cancer cells determined CLU, SRGN and TGFβ2 as significant factors in bacterial cancer targeting. By validation test, clusterin (CLU) was found as a key chemo attractants for E. coli to target lung cancer.

      Conclusion:
      CLU, which released by lung cancer cells, was found as a key regulator for the chemotaxis of E. coli in targeting lung cancer.