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Nozomu Motono
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P3.02 - Biology/Pathology (ID 620)
- Event: WCLC 2017
- Type: Poster Session with Presenters Present
- Track: Biology/Pathology
- Presentations: 1
- Moderators:
- Coordinates: 10/18/2017, 09:30 - 16:00, Exhibit Hall (Hall B + C)
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P3.02-085 - Sphingosine Kinase 1 (SPHK1) Promotes Proliferation and Survival in Non-Small Cell Lung Cancer (ID 7571)
09:30 - 09:30 | Presenting Author(s): Nozomu Motono
- Abstract
Background:
Bioactive sphingolipids, such as sphingosine-1-phosphate (S1P) and ceramide, are signaling molecules involved in the activation of pathways that are directly relevant to carcinogenesis. Sphingosine kinase (SPHK) phosphorylates sphingosine to S1P, and is therefore an important regulator of the levels of ceramide, sphingosine, and S1P. Moreover, SPHK1 has been found to up-regulated in several tumor types, such as glioblastoma multiform, intestinal adenoma, acute erythroleukemia, prostate cancer, gastric cancer, and colon cancer. However, the relationship between bioactive sphingolipid pathway and lung cancer has not been clarified.
Method:
Paraffin-embedded non-small cell lung cancer (NSCLC) samples were obtained from 53 patients diagnosed between June 2009 and May 2014 at the Department of Thoracic surgery, Kanazawa Medical University. We characterized the SPHK1 expression in NSCLC by immunohistochemistry (IHC) and western blotting.
Result:
The SPHK1 expression was revealed at invasive area of NSCLC, and at fibroblast by IHC. The specific band of SPHK1 was detected approximately 52kDa by western blotting. We found that the SPHK1 expression in adenocarcinoma positively correlated with fibroblast expression and Ki-67 expression. On the other hand, the SPHK1 expression in squamous cell carcinoma was not significantly correlated with fibroblast score and Ki-67 expression. Low expression of the SPHK1 tended to prolong the relapse free survival (RFS) in lung adenocarcinoma patients (3-year RFS 100% vs 68.7%, p=0.38) . Figure 1
Conclusion:
SPHK1 expression might regulate proliferation of cancer cell, as well as Ki-67. Furthermore, SPHK1 expression might activate fibroblast which is considered to induce epithelial mesenchymal transition.
