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  P3.03 - Poster Session with Presenters Present (ID 473)

  • Event: WCLC 2016
  • Type: Poster Presenters Present
  • Track: Mesothelioma/Thymic Malignancies/Esophageal Cancer/Other Thoracic Malignancies
  • Presentations: 1
  • Moderators:
  • Coordinates: 12/07/2016, 14:30 - 15:45, Hall B (Poster Area)

  • 18626

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    P3.03-020 - Cytotoxicology Assessment of MWCNT on MeT-5A Cells (ID 6006)

    14:30 - 14:30  |  Author(s): J.L. Lou
    • Abstract
    • Slides

    Background:
    Muti-walled carbon nanotube (MWCNT) is widely used worldwide, but reports already show MWCNT is toxic to experimental animals and cell lines, even causes severe caner, such as mesothelioma. The mechanism of MWCNT toxicities is not very clearly. So, in this study, some toxic effects of MWCNT on MeT-5A cells are evaluated, to offer inclues for futher study.
    
    Methods:
    Cell survival rate was detected by LDH, and cell cycle/ cell apoptosis were measured by flow cytometry. Cell scratch assay was used to evaluate the cell migration capacity.
    
    Results:
    The cytotoxicity of MWCNT on MeT-5A cells are dose- and time-dependent. Cell cycle are blocked in G1/G2 phase, cells in S phase are reduced. The same as cell proliferation, the apoptosis of MeT-5A cells is also time-dependent. MeT-5A cells display a significant reduction of cell migration after MWCNT exposure for 24h and 48h.
    
    Conclusion:
    The cytotoxicity of MWCNT on MeT-5A cells are dose- and time-dependent and the cellular behavior are perturbed by MWCNT treatments.
    
    

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