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L. Zhu
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P3.03 - Poster Session with Presenters Present (ID 473)
- Event: WCLC 2016
- Type: Poster Presenters Present
- Track: Mesothelioma/Thymic Malignancies/Esophageal Cancer/Other Thoracic Malignancies
- Presentations: 1
- Moderators:
- Coordinates: 12/07/2016, 14:30 - 15:45, Hall B (Poster Area)
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P3.03-011 - Downregulated Expression of miR-30d Contributes to Pleural Malignant Mesothelioma Progression (ID 5418)
14:30 - 14:30 | Author(s): L. Zhu
- Abstract
Background:
Pleural malignant mesothelioma(MM) is a highly aggressive tumor that mainly related to asbestos exposure. Some microRNAs (miRNAs) contribute to MM initiation and progression, but the exact mechanism remains largely unknown.
Methods:
The expression of miR-30d in mesothelioma cell line NCI-H2452, chrysotile exposed MeT-5A cells and plasm of 78 human subjects with asbestos exposure was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). After NCI-H2452 cells were transfected with mimics miR-30d to overexpress mir-30d, cell viability was measured by MTS method; in vitro cell migration and invasion was measured by transwell assay and scratch assay; cell cycle and apoptosis was examined by flow cytometry; the mRNA and protein expressions of VIM, TWIST1 and CDH1 were detected by qPCR and western blotting, respectively.
Results:
miR-30d was significantly downregulated not only in NCI-H2452 cells compared with MeT-5A cells, but also in chrysotile exposed MeT-5A cells compared with the negative control and in plasm of asbestos exposed subjects compared with unexposed subjects. After transfected by miR-30d, the proliferation rate, migration rate and invasion cells of NCI-H2452 cells decreased, early apoptosis rate and total apoptosis rate increased in miR-30d transfected NCI-H2452 cells. The mRNA and protein expression of VIM and TWIST1 decreased and the mRNA expression of CDH1 increased by overexpressed miR-30d in NCI-H2452.Figure 1
Conclusion:
Downregulation of miR-30d is related to malignant mesothelioma and asbestos exposure. miR-30d might be a tumor-suppressor miRNA, and its down-regulation might contribute to pleural MM progression and asbestos carcinogenesis.
