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L.R. Teixeira
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P3.02c - Poster Session with Presenters Present (ID 472)
- Event: WCLC 2016
- Type: Poster Presenters Present
- Track: Advanced NSCLC
- Presentations: 1
- Moderators:
- Coordinates: 12/07/2016, 14:30 - 15:45, Hall B (Poster Area)
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P3.02c-009 - Anti-VEGF and Anti-EGFR Reduce Malignant Pleural Effusion and Morbidity in an Experimental Adenocarcinoma Model (ID 5501)
14:30 - 14:30 | Author(s): L.R. Teixeira
- Abstract
Background:
Lung cancer is a main cause of death by cancer worldwide and adenocarcinoma the most common cell type. Most of patients present pleural effusion at an advanced stage of the disease with high morbidity and mortality, however its pathogenesis is still poorly understood and therapeutic options are limited. OBJECTIVE: Evaluate the effects of intrapleural anti-VEGF and anti-EGFR in malignant pleural effusion induced in an experimental model.
Methods:
One hundred and twenty C57BL/6 mice received intrapleural injection of 0.5x10[5] of LLC cells and were divided into four groups that received, after 3, 7, 10 and 14 days, anti-VEGF, anti-EGFR, anti-VEGF+anti-EGFR or PBS (control) intrapleurally. Ten animals for each group were followed until death to evaluate the survival curve. Eighty animals were euthanized after 7, 10, 14 or 21 days after LLC injection and had weight (g), mobility (score 0-3), pleural fluid volume evaluated. Presence of tumor in pleura and pericardium, inflammatory cells in lung parenchyma, histological changes in kidney, liver and spleen and tumor apoptosis (TUNEL) and proliferation (PCNA) were evaluated by score (0-4). Statistical analysis: One Way ANOVA, Kaplan–Meier curve, p<0.05.
Results:
In the survival analysis, pleural carcinomatosis was lethal showed maximum survival of 25 days without statistical differences among groups (p=0.739). Reduction of body weight mice was observed in all groups after 21 days (p<0.05). However, the animals mobility was better in the groups that received anti-EGFR (p=0.026). The fluid volume was higher in all control groups no matter the study time (p=0.010). Tumor implants in the pleura were more evident in control groups compared to treated groups after 14 days (p=0.001). Neoplastic infiltration of lung parenchyma was observed only in a few animals. However, lung parenchymal inflammation was minimal in all groups. Histological evaluation of pericardium and heart muscle showed tumor implants mainly in the 21-day in the control group. In liver and kidney steatosis were observed after 14 days in control group (p<0.001). Hyperplasia of the white pulp of the spleen was observed at all evaluation time points with greater evidence at 21-day in the control group (p<0.001). High scores of apoptosis and lower scores of tumor proliferation were observed in the groups that received treatment with anti-EGFR and anti-VEGF+antiEGFR.
Conclusion:
In this experimental model, the target therapies reduced significantly the pleural fluid volume, morbidity and histological parameters mainly in the therapies with EGFR, although its action did not increased the survival of the animals.
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P3.04 - Poster Session with Presenters Present (ID 474)
- Event: WCLC 2016
- Type: Poster Presenters Present
- Track: Surgery
- Presentations: 1
- Moderators:
- Coordinates: 12/07/2016, 14:30 - 15:45, Hall B (Poster Area)
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P3.04-034 - Differences between Pleurodesis Using Talc and Silver Nitrate at Different Times of Pleural Disease in Mice (ID 5340)
14:30 - 14:30 | Author(s): L.R. Teixeira
- Abstract
Background:
Recurrent malignant pleural effusion occurs in approximately 50% of patients with metastatic tumors and their therapy is essentially palliative. The most used method is the chemical pleurodesis. However, we don’t know what would be the ideal time to submit the patient to the procedure, neither what the best sclerosing agent. The objective is to analyze if the progression of the pleural neoplastic disease is associated with the degree of fibrosis in mice subjected to pleurodesis with talc and nitrate, in animals injected with 10 thousand Lewis’s cells intrapleural.
Methods:
In this experimental study we used twenty C56-BL mice, with pleural cancer induced by injection of 10.000 Lewis cells/ml of 0.9% saline. On the third day of pleural disease, half of the animals were subjected to plerodese, 5 of them with talc at a concentration of 400mg/kg(called Group 3 Talc-"GT3") and other 5 with silver nitrate in a concentration of 0.05%(called nitrate Group-"GN3"). On the seventh day of pleural disease, the remaining subjects were again divided into 2 groups of 5 animals(GT7andGN7) and subjected to plerodesis with the same substances and concentrations. All animals were sacrificed 7 days after pleurodesis, regardless of which group they belonged.
Results:
All animals were pleural implants of cancer cells. Regarding the macroscopic findings were graded fibrosis in score been validated in other studies ranging from 0(no fibrosis) to 4(complete symphysis). In GT3 group, 2 animals had a score 2, and 2 animals obtained score 0.5. In the nitrate group(GN3) 3 animals had a score 0. In animals underwent pleurodesis after 7 days of illness, the talc group(GT7), 3 animals received a score of 0,2 and 2.5 respectively; the nitrate group(GN7) received scores of 0.5, 1 and 2. Microscopically, all had the presence of fibroblasts and fibrosis on visceral pleura. 2 blades each group(GT3 GN3, and GT7 GN7) were stained with picrosirius method for evaluating local fibrosis, and all had positive staining method including quantitation amount sufficient to further study. The animal control cutting blade, with only pleural disease had negative results.
Conclusion:
Animals with this number of implanted cells have sufficient survival and satisfactory answer to pleurodesis so we can quantify it according to the available methods. There was no weight loss or significant reduction in activity of the animals during time. Apparently, there is a greater amount of fibrosis in animals submitted to pleurodesis with talc.
