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I. Tatsuya
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P3.01 - Poster Session with Presenters Present (ID 469)
- Event: WCLC 2016
- Type: Poster Presenters Present
- Track: Biology/Pathology
- Presentations: 1
- Moderators:
- Coordinates: 12/07/2016, 14:30 - 15:45, Hall B (Poster Area)
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P3.01-046 - Klotho Regulates Epithelial-Mesenchymal Transition in Lung Squamous Cell Carcinoma (ID 5240)
14:30 - 14:30 | Author(s): I. Tatsuya
- Abstract
Background:
Klotho gene was known as one of the anti-aging gene. We previously reported that the expression of the Klotho gene was an important postoperative prognosticator for lung large cell neuroendocrine carcinoma and lung small cell carcinoma. Recently, it has been shown that the Klotho gene suppresses epithelial-mesenchymal transition (EMT). In this study, we examined the association between the expression of Klotho and the regulation of EMT in lung squamous cell carcinoma.
Methods:
We examined the expression of Klotho in patients with lung squamous cell carcinoma, who received surgical resection or photodynamic therapy, by immunohistochemical analysis. In order to elucidate the association between the expression of Klotho and expression of EMT related protein, such as E-cadherin, N-cadherin, Vimentin and Snail, we transfected GFP-Klotho plasmid DNA into human squamous lung cancer cell line SQ5. Twenty four hours later, we sorted GFP-positive cells by flowcytometry using FACSCantoII (BD Biosciences, CA, USA), and then we examined the protein levels by Western blot analysis.
Results:
By immunohistochemical analysis, Klotho expression was observed in not only normal bronchial epithelial cells but also centrally located early lung cancers, which were all carcinoma in situ and treated by PDT. However, in lung cancer patients with invasive and or advanced squamous cell carcinoma who received surgical resection completely, Klotho expression was observed in only 4 patients (13%). In SQ5 cells transiently overexpressing GFP-Klotho, the expression of N-cadherin,which is one of mesenchymal markers, was completely inhibited compared with the SQ5 cells transfected with GFP vector. Overexpression of Klotho affected the regulation of neither other mesenchymal markers such as Vimentin and Snail nor epithelial marker, E-cadherin.
Conclusion:
We conclude that the expression of Klotho was related to the cancer invasive ness and Klotho inhibited the expression of N-cadherin, and regulates the EMT in lung cancer. Klotho may play an important role in cancer treatment and molecular-targeted therapy.
