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K. Okuda
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P3.01 - Poster Session with Presenters Present (ID 469)
- Event: WCLC 2016
- Type: Poster Presenters Present
- Track: Biology/Pathology
- Presentations: 1
- Moderators:
- Coordinates: 12/07/2016, 14:30 - 15:45, Hall B (Poster Area)
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P3.01-044 - Splicing Variant of Estrogen Receptor Alpha is Associated with Pathological Invasiveness in Smoking Independent Lung Cancer (ID 4863)
14:30 - 14:30 | Author(s): K. Okuda
- Abstract
Background:
Smoking independent lung cancers are consisted mainly of female patients, but the molecular background of this epidemiological feature other than EGFR mutation is still vague. Several studies have reported the correlation between female hormone related factors and the prognosis of lung cancer, but the results are still inconsistent. We focused on the expression of aromatase, estrogen receptor alpha (ER alpha), and estrogen receptor beta (ER beta) to investigate the carcinogenesis of smoking independent lung cancer.
Methods:
Immunohistochemistry staining (IHC) of aromatase, ER alpha, and ER beta was performed against formalin fixed tissues from 38 never-smoking patients who underwent complete surgical resection between 2012 and 2013. Among them, adequate RNA of the tumor and adjacent normal lung were extracted from deep frozen tissues of 31 patients. Considering the IHC results, quantitative RT-PCR (qRT-PCR) was performed to measure the expression level of aromatase and 3 different exons of ER alpha (exon4-5, exon6, and exon7) which composes the ligand binding motif using the Taqman© method.
Results:
Extra-nuclear expression of ER alpha with IHC showed significant correlation with pathological invasiveness, statistically. qRT-PCR results showed decreased expression of ER alpha exon 7 in invasive tumor tissues, compared with their adjacent normal tissues. This is consistent with previous in vitro results indicating that extra-nuclear ER alpha were exon7 splicing variants. There was no difference of ER alpha exon7 expression between normal and tumor tissues in non-invasive lung cancer tissues. When considering EGFR mutation status, EGFR wild type lung cancers showed lower ER alpha exon7 expressions compared with EGFR mutated lung cancers.
Conclusion:
Extra-nuclear expression of ER alpha, which may represent exon7 splicing variants of ER alpha, correlates with pathological invasiveness in smoking independent lung cancer. It may also have a part in carcinogenesis of EGFR wild type lung cancer.
