Author of

• 18272

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  P3.01 - Poster Session with Presenters Present (ID 469)

  • Event: WCLC 2016
  • Type: Poster Presenters Present
  • Track: Biology/Pathology
  • Presentations: 1
  • Moderators:
  • Coordinates: 12/07/2016, 14:30 - 15:45, Hall B (Poster Area)

  • 18312

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    P3.01-040 - Difference of Graphene Oxide-Induced Autophagy between Adenocarcinoma and Macrophage Cell Line (ID 4482)

    14:30 - 14:30  |  Author(s): S.Y. Kim
    • Abstract

    Background:
    Chemotherapy against nonsmall cell lung cancers is remarkably progressing. Nanomaterials are searched for this therapy. Graphene oxide is also suggested as one of promising therapeutic materials. Graphene is an allotrope of carbon with honeycomb structure. It may show the diverse biologic effects from minimal to highly toxic effect according to the cell types.The goal of this study was to define the differential cell death mechanism of graphene oxide on lung adenocarcinoma cells and macrophages with focusing autophagy.
    
    Methods:
    A549 cells and Raw264.7 cells were cultured in Dulbecco's modified eagle's medium with 10 % fetal bovine serum and treated with graphene oxide(GO). GO was treated to the cells in the range of 5 ~ 200 ug/ml for 24 and 48 hours. Cell survival was examined with light microscopy and MTT assay. Protein expression was checked by Western blots for LC3A/B-I, II, NBR1, p62/SQSTM1, mTOR, Bec-1 and PU.1(monocyte/macrophage-specific transcription factor).
    
    Results:
    Higher concentrations of graphene oxide induced increasing cellular death with different intensity between A549 and Raw264.7 cells. LC3B-I to II conversion (autophagy) was increased by GO in A549 cells and decreased in Raw264.7 cells. Expression of NBR1 and p62 showed same directional change in both cell types. The mammalian TOR was also decreased in A549 cells. PU.1(monocyte/macrophage-specific transcription factor) was decreased in Raw264.7 cells. Bec-1 and GAPDH was not affected by GO in both cells.
    
    Conclusion:
    This study showed the opposite response in autophagy in A549 cells and Raw264.7 cells. Although the precise mechanisms are mandatory to be defined, graphene may be used for selective targeting against lung adenocarcinoma with preserving immune function.