Author of

• 18272

  +

  P3.01 - Poster Session with Presenters Present (ID 469)

  • Event: WCLC 2016
  • Type: Poster Presenters Present
  • Track: Biology/Pathology
  • Presentations: 1
  • Moderators:
  • Coordinates: 12/07/2016, 14:30 - 15:45, Hall B (Poster Area)

  • 18309

    +

    P3.01-037 - The Role of HIF3A Polymorphism in Lung Cancer Patients (ID 3933)

    14:30 - 14:30  |  Author(s): A.C. Putra
    • Abstract

    Background:
    Hypoxia-inducible factor (HIF) is important for cancer progression, resistance to therapy and development of cancer itself. A family of transcription factors HIF is heterodimer consist of α subunits (HIF-1α, HIF-2α, HIF-3α), which forms an active complex with β subunits also known as the aryl hydrocarbon nuclear translocation (ARNT) then stimulate various target genes, termed as hypoxia response element (HRE). However, a genetic variant of HIF was not fully understood. Previously, we reported that HIF1A polymorphism associated with TP53 status in lung cancer patients, and transcriptional activity of the HIF-1α variants in A549 lung cancer cells was significantly greater than that of the wild type, especially in cells containing a mutant type of p53. We also found that one of HIF2A (EPAS1) polymorphism significantly associated with poorer prognosis of lung cancer patients, and the nucleotide substitution might affect HIF2A expression through transcriptional regulation in vitro.
    
    Methods:
    In this study, we tried to clarify a role of genetic variations of HIF3A gene, and started evaluations of six polymorphisms located in HIF3A loci (rs3764609, rs3764610, rs3764611, rs375220, rs3810302, rs3826796) in 83 Japanese lung cancer patients as a pilot study.
    
    Results:
    We performed sequence analysis of genomic DNA and success identify HIF3A polymorphisms by direct sequencing. Genotype distributions of each SNP showed good agreements with the Hardy-Weinberg equilibrium. We found the rs3810302 have different genotype distribution compare healthy Japanese data base (HapMap), p=0.011. Then, some loci of HIF3A showed significant associated with clinicopathological of lung cancer patients (stage, cancer differentiation, histology, etc).
    
    Conclusion:
    Our preliminary study suggested that some of HIF3A polymorphisms showed significantly important associations with lung cancer clinicopathological. More studies were further required to focus on its relationship.