Author of

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  P3.01 - Poster Session with Presenters Present (ID 469)

  • Event: WCLC 2016
  • Type: Poster Presenters Present
  • Track: Biology/Pathology
  • Presentations: 1
  • Moderators:
  • Coordinates: 12/07/2016, 14:30 - 15:45, Hall B (Poster Area)

  • 18275

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    P3.01-003 - Thyroid Transcription Factor-1 (TTF-1) Expression for Intraoperative Diagnosis Using the Rapid-Immunohistochemistry (IHC) in Lung Tumors (ID 3943)

    14:30 - 14:30  |  Author(s): Y. Minamiya
    • Abstract

    Background:
    Thyroid transcription factor-1 (TTF-1) is useful as an immunohistochemical marker of the high specificity of the primary lung adenocarcinoma. Whereas immunohistochemistry (IHC) is emerging as a powerful tool in undetermined diagnosis case, it was difficult to achieve intraoperative quickly IHC due to time constraints. We previously developed and reported a device that enables us to complete IHC analyses within 20 minutes. The purpose of rapid-IHC analysis during surgery is more accurate tissue diagnosis. Because of lung adenocarcinoma discrimination, we were examined the usefulness of TTF-1 rapid IHC.
    
    Methods:
    Eighty-two patients with lung tumor were enrolled in the study between May 2011 and September 2013. Resected samples from each patient were sectioned immunohistochemically labeled with anti-TTF-1 antibody using the rapid-IHC procedures.
    
    Results:
    Using the rapid-IHC procedure, IHC analyses were completed within 20 min, and TTF-1-positive tumors were detected by the pathologist within about 30 min. Intraoperative diagnosis using the rapid-IHC procedure was resulting in 97.6% accuracy (80/82). Of the 47 primary lung adenocarcinoma eligible for analysis, 31 (66%) were positive for TTF-1 by intraoperative diagnosis using the rapid-IHC procedure, and the positive predictive value (PPV) of primary lung adenocarcinoma was 100% (31/31).
    
    Conclusion:
    Our experience demonstrates that intraoperative IHC investigation of TTF-1 using the rapid-IHC in proper consequences could be a very useful tool for lung tumors. Further investigation in multicenter studies　will be needed to confirm the utility of this method. With refinement, this technology may prove to be an important supplement to standard pathology for routine practice.