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R. Hu
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P2.05 - Poster Session with Presenters Present (ID 463)
- Event: WCLC 2016
- Type: Poster Presenters Present
- Track: Radiotherapy
- Presentations: 1
- Moderators:
- Coordinates: 12/06/2016, 14:30 - 15:45, Hall B (Poster Area)
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P2.05-027 - Effects of Thermo-Chemotherapy for Lung Cancer Induced by Nano-Paclitaxel Magnetic Fluid (ID 3781)
14:30 - 14:30 | Author(s): R. Hu
- Abstract
Background:
The aim of this study was to investigated the effects of thermo-chemotherapy induced by nano-paclitaxel magnetic fluid for lung cancer A549 proliferation, apoptosis and cell cycle in vitro, and therapeutic effect of human carcinoma A549 xenograft in nude mice in vivo.
Methods:
In vitro, nano-paclitaxel magnetic liquid was synthesised by chemical coprecipitation and ultrasound emulsification. Lung cancer A549 cells were set up the control group (group A), thermal therapy group (group B), chemotherapy group (group C) and thermo-chemotherapy group (group D), which exposed to an alternative magnetic field (AMF) for 30 min. And then the optical density (OD) of viable cell, cytotocixity index, growth curve of cells, morphologic changes of cell, cell cycle and aposptosis were measured. When tumor length to diameter (6 ~ 8 mm), they were randomly divided into 4 groups: control group, magnetic heat treatment group, paclitaxel magnetic thermo-chemotherapy group and chemotherapy group, the tumor was heated in an AMF for 30 min. Tumor volumes were then measured every week. The therapeutic effect was assessed by measuring the tumor volume and weight. Pathological examination was performed with a light microscope following treatment. Immunohistochemical detecting tumor after treatment tumor cell apoptosis, calculate the apoptosis index to compare the efficacy of treatment.
Results:
In 43 ℃, with the increase of paclitaxel concentrations, are more obvious A549 lung cancer cell proliferation inhibition, the number of cells in living cells of optical density value, the killing rate (cytotoxity index, CI). Cell apoptosis rate increased. Heat treatment group the stagnation of the cell cycle in S phase, S phase cells and G2 phase increases, S phase decreased in the chemotherapy group, after heat treatment of lung cancer cells in electron microscope magnetic apoptotic changes. The temperature inside the tumor can be quickly rise to 43 ℃. Tumors in three experimental groups are suppressed, magnetic thermo-chemotherapy group tumor growth inhibition is more obvious, immunohistochemical confirmed the tumor cell apoptosis in change, apoptosis index increased.
Conclusion:
In vitro, with the increase of paclitaxel concentrations, are more obvious A549 lung cancer cells proliferation inhibition in 43 ℃. The number of cells in living cells of optical density value, the killing rate (cytotoxity index, CI), cell apoptosis rate increased. Thermo-chemotherapy induced by nano-paclitaxel magnetic fluid can inhibit the growth of A549 lung cancer nude mice transplantation tumor, nano paclitaxel magnetic thermo-chemotherapy can enhance the anti-tumor effect in vivo.
