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A. Rice
Author of
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P2.03b - Poster Session with Presenters Present (ID 465)
- Event: WCLC 2016
- Type: Poster Presenters Present
- Track: Advanced NSCLC
- Presentations: 1
- Moderators:
- Coordinates: 12/06/2016, 14:30 - 15:45, Hall B (Poster Area)
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P2.03b-086 - High Expression of PDL-1 Correlates with Pleomorphic Features in Non-Small Cell Lung Carcinomas (ID 5761)
14:30 - 14:30 | Author(s): A. Rice
- Abstract
Background:
With immunomodulatory therapy being integrated into treatment regimes for non-small cell carcinoma (NSCLC), we have reviewed our initial experience within the UK in relation to potential access to treatment with pembrolizumab, in order to assess correlation between tumour morphology and staining patterns.
Methods:
Immunohistochemistry for PD-L1 (IHC/PD-L1) was performed with the PD-L1 IHC 22C3 pharmDxTM assay (Dako) on cases being considered for treatment. The test was considered adequate when more than 100 tumour cells were seen microscopically. When adequate, PD-L1 staining was scored as 0%, <1%, ≥ 1-49% or ≥ 50% positive membrane staining within tumour cells only. PD-L1 staining was considered positive when the score was >50%. In initial cases, it was noticed that there was increased staining in cases with pleomorphic features, so a separate cohort of 9 resections of pleomorphic carcinomas was additionally assessed.
Results:
PD-L1 expression was assessed in 72 NSCLC test cases which comprised 19 lung resections, 31 lung biopsies, 11 lymph node biopsies (5 of which were TBNAs), 4 pleural/pericardial tissue (2 from effusions), and 7 other metastatic sites (cores). There were 52 ADC (8 of which were NSCLC-ADC on IHC and 3 of which were invasive mucinous ADC), 7 SQCC (2 of which were NSCLC-SQCC on IHC), one LCC, 2 NSCLC-NE and 4 NSCLC-NOS. 6 cases were inadequate. Of the 65 cases with adequate tissue, 9 cases had pleomorphic features. A score of >50% was found in 78% (7/9) of cases with pleomorphic features with the remainder being 1-49%, compared to 27% (15/56) in those without pleomorphic features (p<0.05) (1-49% = 22 ,<1% = 19). 8 of 9 (89%) additional resected pleomorphic carcinomas showed >50% positivity with one case showing 10% positive staining concentrated in the pleomorphic area.
Conclusion:
Initial data show a correlation between PD-L1 staining and pleomorphic features in non-small cell lung carcinomas. Assessment on cell blocks obtained from TBNAs and effusions also provide assessable material.
