Author of

• 17629

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  P2.03b - Poster Session with Presenters Present (ID 465)

  • Event: WCLC 2016
  • Type: Poster Presenters Present
  • Track: Advanced NSCLC
  • Presentations: 1
  • Moderators:
  • Coordinates: 12/06/2016, 14:30 - 15:45, Hall B (Poster Area)

  • 17703

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    P2.03b-074 - NSCLC Homing Nanoparticles Selectively Transfect Lung Cancer (ID 5698)

    14:30 - 14:30  |  Author(s): G. Holt
    • Abstract

    Background:
    Gene therapy could treat lung cancer by transfecting cancer cells with plasmid DNA encoding the genes of interest. Unfortunately, it is not clinically feasible to perform repetitive intratumoral injections due to the anatomic location of lung cancer.
    
    Methods:
    To circumvent this problem, we created a dendrimer consisting of a fifth generation nanoparticle attached to a peptide with binding specificity for non-small cell lung cancer called NSCLC-NP.
    
    Results:
    NSCLC-NP shows selective binding and transfection of human lung cells in vivo. In vivo, systemically applied NSCLC-NP home to human lung cancer cells growing in RAG2 KO mice and cause expression of the gene encoded by the attached plasmid. Dendrimers can accumulate at tumors due to the Enhanced Permeability and Retention effect. Using a corneal model growing human lung cancer cells, NSCLC-NP show binding to human lung cancer cells is based on the lung cancer homing peptide and not the EPR.
    
    Conclusion:
    The NSCLC-NP reagent is a vehicle to cause expression of any gene by lung cancer cells after systemic administration.