Author of

• 17398

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  P2.01 - Poster Session with Presenters Present (ID 461)

  • Event: WCLC 2016
  • Type: Poster Presenters Present
  • Track: Biology/Pathology
  • Presentations: 1
  • Moderators:
  • Coordinates: 12/06/2016, 14:30 - 15:45, Hall B (Poster Area)

  • 17405

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    P2.01-007 - Detection of Promoter DNA Methylation of APC, DAPK, and GSTP1 Genes in Tissue Biopsy and Matched Serum of Advanced Stage Lung Cancer Patients (ID 5595)

    14:30 - 14:30  |  Author(s): V.K. Kakaria
    • Abstract

    Background:
    Promoter DNA hypermethylation is a well characterized epigenetic event and has been linked with early stages of lung carcinogenesis through inactivation of tumor suppressor genes. In this study, we studied the methylation status of APC, DAPK, and GSTP1 genes in tissue biopsy and serum of lung cancer patients and cancer-free controls.
    
    Methods:
    In this prospective study, 160 primary lung cancer patients and 70 cancer-free controls undergoing bronchoscopy for benign disease were recruited. DNA was isolated from tissue biopsy and serum of all the subjects and methylation-specific PCR of APC, DAPK, and GSTP1 was carried out after bisulfite conversion. Association of DNA methylation with various clinico-pathological parameters and survival was determined in lung cancer patients.
    
    Results:
    The methylation rates of APC, DAPK, and GSTP1 in tissue biopsy were 83.1%, 83.1%, and 78.1% for lung cancer patients and 72.9%, 70%, and 70% for cancer-free controls. The methylation rates of APC, DAPK, and GSTP1 in serum were 52.5%, 30.6%, and 65.6% for lung cancer patients and 14.3%, 18.6%, and 30% for cancer-free controls. In lung cancer patients, all three genes were methylated at significantly higher frequency in tissue biopsy than matched serum samples. No significant correlation was observed between methylation of any of three genes with clinico-pathological parameters, including survival.
    
    Conclusion:
    Present study did not demonstrating any evidence suggesting the role of promoter DNA methylation of APC, DAPK, and GSTP1 in lung carcinogenesis. However, follow-up of cancer-free controls, who were positive for DNA methylation, is required to confirm their role in early stages of lung carcinogenesis.