Author of

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  P1.05 - Poster Session with Presenters Present (ID 457)

  • Event: WCLC 2016
  • Type: Poster Presenters Present
  • Track: Early Stage NSCLC
  • Presentations: 1
  • Moderators:
  • Coordinates: 12/05/2016, 14:30 - 15:45, Hall B (Poster Area)

  • 16769

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    P1.05-018 - LncRNA16 is a Potential Biomarker for Early-Stage Lung Cancer That Promotes Cell Proliferation by Regulating the Cell Cycle (ID 4693)

    14:30 - 14:30  |  Author(s): H. Zhu
    • Abstract
    • Slides

    Background:
    Early diagnosis of lung cancer greatly reduces mortality; however, the lack of suitable plasma biomarkers presents a major obstacle. Recent studies showed that long noncoding RNAs (lncRNAs) play important roles in cancer initiation and development.
    
    Methods:
    Here, we identify differential expressed lncRNAs by using custom designed microarray on 20 lung cancer samples and evaluate the expression by Real-time PCR (qRT-PCR) on 118 lung cancer samples.The role of lncRNA16 in lung cancer was studied in vitro and in vivo, utilizing the lung cancer cell line PC9 ,A549 and xenograft mouse models.
    
    Results:
    lncRNA16 (ENST00000539303) expression level was highly in lung cancer (80/118) and in plasma (32/42) of lung cancer patients. In early stage, lncRNA16 expression levels were significantly higher compare to that in adjacent matched normal tissues (Figure 1C-1F) . Importantly, this increase was mirrored in plasma samples of early stage lung cancer patients (Figure 2A) . Our study reveals that knockdown of lncRNA16 inhibited proliferation of PC9 cells in vitro and also inhibited tumor growth in xenograft mouse models. Specifically, we show that lncRNA16 promotes G2/M transition through regulating cyclin B1 transcription.
    
    Conclusion:
    In conclusion, lncRNA16 was identified as a potential biomarker for lung cancer diagnosis, as it displayed significantly elevated levels in cancer patient over baseline. Furthermore, we showed that the false-negative rate is significantly lower compared to markers those widely used for lung cancer assessment.
    
    

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