Author of

• 16751

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  P1.05 - Poster Session with Presenters Present (ID 457)

  • Event: WCLC 2016
  • Type: Poster Presenters Present
  • Track: Early Stage NSCLC
  • Presentations: 1
  • Moderators:
  • Coordinates: 12/05/2016, 14:30 - 15:45, Hall B (Poster Area)

  • 16766

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    P1.05-015 - Genomic Characterisation of Non-Small Cell Lung Cancer in an Australian Population (ID 4052)

    14:30 - 14:30  |  Author(s): D. Irwin
    • Abstract

    Background:
    Lung cancer is a heterogeneous disease with poor prognosis. Genomic variants may predict sensitivity to targeted drug therapies or assist in prognostication. We sought to determine the frequency of driver mutations and gene rearrangements in non-small cell lung cancer (NSCLC) and evaluate the feasibility of the MassARRAY system for multiplexed mutational profiling.
    
    Methods:
    A cohort study of 419 fresh-frozen NSCLC tumours was performed (AC, n=370; SCC, n=39; ASC, n=7; LCC, n=3). High-throughput and multiplexed mutational profiling was performed using the MassARRAY genotyping system (Agena Bioscience) (n=419). The OncoFOCUS+KIT panel was used for detecting genomic variants in EGFR, BRAF, KRAS, NRAS and KIT (n=413) and the LungFusion panel for fusion genes involving ALK, RET and ROS1 (n=371). Clinico-pathological associations were evaluated using Fisher’s exact test for categorical data, and T test for continuous data. A p-value of <0.05 (two-tailed) was considered statistically significant.
    
    Results:
    At least one genomic variant was detected in 196 (46.8%) cases (n=419). EGFR mutations were identified in 42 cases (10.2%), KRAS in 133 (32.3%), BRAF in 11 (2.7%), NRAS in 4 (1.0%) and no KIT mutations were detected. Gene rearrangements involving ALK, RET and ROS1 were identified in 2 (0.5%), 1 (0.3%) and 5 (1.3%) cases respectively. Based on current clinical guidelines for NSCLC, 28 patients would qualify for tyrosine kinase inhibitor therapy, and 4 for targeted therapy available for other cancers (BRAF V600E). EGFR mutations were significantly associated with adenocarcinoma histology and female never smokers (p<0.001) and KRAS mutations predominated in smokers (p<0.001).
    
    Conclusion:
    Driver mutations were detected in 46.8% of NSCLC cases resected at TPCH. Rapid, multiplexed mutation testing can guide treatment as well as assist in patient stratification for clinical trials.