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D.A. Nader
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P1.04 - Poster Session with Presenters Present (ID 456)
- Event: WCLC 2016
- Type: Poster Presenters Present
- Track: Pulmonology
- Presentations: 1
- Moderators:
- Coordinates: 12/05/2016, 14:30 - 15:45, Hall B (Poster Area)
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P1.04-019 - Final Analysis of Lung Microbiome from Patients Undergoing Bronchoscopy (ID 4201)
14:30 - 14:30 | Author(s): D.A. Nader
- Abstract
Background:
Recent studies have demonstrated diversity in the lung microbiomes of chronic obstructive pulmonary disease and healthy individuals. Lung microbial communities may not just serve as a predictor of cancer development, but also as a target of pharmacological cancer prevention strategies. We sought to characterize the lung microbiome diversity within patients with lung cancer for comparison to those with other cancers and those without cancer.
Methods:
Signed informed consent was obtained from patients ages ≥18 years undergoing a clinically indicated bronchoscopy. A bronchial lavage (BAL) was collected for research purposes after completing routine bronchoscopic procedures. Samples were prepped and DNA was extracted and 515F/806R 16S rRNA primers used to amplify Variable Region 4. Amplicons were sequenced and grouped into 100% operational taxonomic units (OTUs) using vsearch. Taxonomy was assigned, a phylogenetic tree was constructed, and sequences aligned for phylogenetic diversity calculations, including Faith's Phylogenetic Diversity and weighted and unweighted UniFrac. OTUs that were significantly different across sample categories were identified using DESeq2.
Results:
There were 137 unique BAL samples collected. One patient had an adverse research procedure event that resolved after temporary supplemental oxygen and overnight observation. BALs were from 68 non-small cell lung cancer (NSCLC), 12 small cell lung cancer (SCLC), 52 other cancers, and 5 non-cancer patients. 58 NSCLC were current/former smokers (average 43 pack-years), while all the SCLC were current/former smokers (average 56 pack-years). 22 other cancers were current/former smokers (average of 27 pack-years). Overall, 51 samples (37.2%) had sufficient sequencing reads (>20,000) for subsequent analyses. There were multiple bacterial taxonomic groups in each sample, however, phylogenetic diversity was low compared to other body sites. There were no statistical differences in alpha/beta diversity between ever-smokers and never-smokers, NSCLC vs SCLC, lung cancer vs non-cancer, and location of BAL collection (upper vs lower airways and right vs left lung). There were a number of statistically significant differences by taxonomy (False Discovery Rate adjusted p<0.01 and listing genus only). Adenocarcinoma vs non-adenocarcinoma had more Streptococcus and Veillonella; less Haemophilus. For NSCLC vs SCLC, Rothia was more prevalent in SCLC. BALs from the upper airways had more prevalent Streptococcus. BALs from the right lung had more prevalent Capnocytophaga and Parvimonas; less Moraxella and Selenomonas.
Conclusion:
We report significant taxonomic differences by tumor type and location of BAL sampling and overall low phylogenetic diversity. Future validation of this work can be used to modify bacterial colonization in a lung cancer prevention strategy or for early diagnostics/therapeutics.
