Author of

• 16549

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  P1.02 - Poster Session with Presenters Present (ID 454)

  • Event: WCLC 2016
  • Type: Poster Presenters Present
  • Track: Biology/Pathology
  • Presentations: 1
  • Moderators:
  • Coordinates: 12/05/2016, 14:30 - 15:45, Hall B (Poster Area)

  • 16634

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    P1.02-085 - Molecular Profile in NSCLC Biopsy Samples: A Multicenter Local Study (ID 6213)

    14:30 - 14:30  |  Author(s): M. Canigiani
    • Abstract

    Background:
    During the last time substantial progress have been made in the characterization of the molecular abnormalities of NSCLC tumors such as activations of oncogenes by mutations, translocations and amplifications, which are being used as molecular targets and predictive biomarkers. Currently these molecular analysis is mandatory for therapy selection.
    
    Methods:
    92 small biopsies and resection specimens of patients with NSCLC (AC) in different institutions of Cordoba were studied during a period (2014 2016). We determined the frequency of molecular alterations in EGFR and gene fusion ALK in our Caucasian and Hispanic populations to decide the adequate treatment . Histopathology Type,immunohistochemistry (IHC) characteristics as well as molecular profile and several clinical variables were studied. To detect alterations of EGFR and fusion gene EML4-ALK expression, different tests were used with the aim to identify our own profile and decide the adequate therapeutical option. EGFR mutation was studied by therascreen kit, PCR, in order to detect genetic alterations in exons 18, 19, 20 and 21. ALK translocations were analyzed by FISH (Vysis- Break Apart, Abbott) and IHC (clon D5F3, ventana, Roche). The molecular profiles were correlated with different clinical variables (age, gender, and tobacco habits). The statistical method used was the multiple regression logistic model.
    
    Results:
    58 men and 34 women out of 92 samples were tested for EGFR expression. Eight men and ten women expressed EGFR positive. Sixteen men and two women were smokers. Activating kinase-domain mutations in EGFR were identified in 21 pts (22,82 %): exon 19 deletion = 11, L858R = 7, exon 20 insertion = 1, other = 2. EGFR alterations were related with gender , women showed more alterations of the genes. Age and smoking habit of patients did not show significant association . We used the multiple regression logistic model to correlate EGFR expression to age, gender, tobacco habits. We identified 4 pts (5%) with fusion gene EML4-ALK. ALK alterations were not related to gender, age and smoking habit .
    
    Conclusion:
    Our results showed a comparable frequency in EGFR mutations and gene fusion ALK in relation to the data published in western population and our data presented in LALCA meeting 2016. These results permit an adequate diagnosis to provide these pts with the most benefitial therapy.