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M. Aguirre
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P1.02 - Poster Session with Presenters Present (ID 454)
- Event: WCLC 2016
- Type: Poster Presenters Present
- Track: Biology/Pathology
- Presentations: 1
- Moderators:
- Coordinates: 12/05/2016, 14:30 - 15:45, Hall B (Poster Area)
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P1.02-053 - Comparison of Two Different Commercially Available Probes for the Detection of ALK Rearrangements in Cytological Smears (ID 6083)
14:30 - 14:30 | Author(s): M. Aguirre
- Abstract
Background:
Many patients with NSCLC are diagnosed at advanced stages. A high percentage of those patients have only cytological samples for morphological and molecular analysis. Furthermore ALK analysis by FISH has to be performed using a specific commercially available probe. The aim of this study is to compare two different commercially available probes for analysis of ALK gene rearrangements by FISH using cytological stained smears.
Methods:
We analyzed 37 cytological stained smears from 37 consecutive cases of FNA (14 Diff-Quick and 23 Papanicolau). ROSE was performed in all procedures. The status of EGFR, KRAS and BRAF was kwon in 28 patients. Two probes were used: LSI ALK BREAK APART (Vysis, Ref: 53206N38020, Izasa) was apply in all 37 cases. Additionally, in 25 of these cases we used the ZytoLight SPEC ALK Dual Color Break Apart (ZYTOVISION, Ref: Z-2124-200, Menarini Diagnostics) probe. Protocols were modified from Basel and Zytolight manufacture guidelines. In order to optimize the amount of probe and to evaluate individual nuclei, we delimited an area on each smear with no nuclear overlap.
Results:
All cases were adenocarcinomas. Two cases showed ALK rearrangement (5.4%). Of the 34 negative cases, 27 were negative-polysomic (77.14%). One case was no assessable (2.7%) due to the impossibility of getting hybridization. Concordance between two probes was 100%. No differences were found between Papanicolau and DiffQuick stained smears .
Conclusion:
FISH-ALK in cytological stained smears gives excellent results. Both commercially avialable probes showed identical performance, both are equally valid. No differences between Papanicolau and Diff-Quick stained smears were observed. In our experience, stained cytological smears are the best choice for the analysis of ALK rearrangements in NSCLC patients, keeping paraffin for cases in which adequate cytological smears are not available.
