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M. Farooq
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P1.02 - Poster Session with Presenters Present (ID 454)
- Event: WCLC 2016
- Type: Poster Presenters Present
- Track: Biology/Pathology
- Presentations: 1
- Moderators:
- Coordinates: 12/05/2016, 14:30 - 15:45, Hall B (Poster Area)
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P1.02-028 - Detection of Oncogenic Drivers in Pleural Effusions and Archived FNA Smears of Pulmonary Adenocarcinoma (ID 5176)
14:30 - 14:30 | Author(s): M. Farooq
- Abstract
Background:
Cytological materials are widely used in diagnosis and staging of lung cancer due to advanced stage of disease at the time of presentation. Mutational oncogenic drivers in pulmonary adenocarcinoma (ADC) include EGFR, Kras and Her-2/neu. We utilized archived FNA smears and pleural effusion samples of ADC for detection of oncogenic molecular drivers.
Methods:
Pleural fluids (36) and May Grunwald stained FNA smears (18) were used for mutation anaysis. Sanger sequencing and ARMS and Scorpions PCR performed. Each tumor sample was evaluated for all classes of genomic alterations, including base-pair substitutions, insertions/deletions, as well as intronic/CDS polymorphisms.
Results:
There were 31 males and 23 females, aged 18 to 82 years with mean age 65.5 years. A total of 9 patients (16.6%) were found positive for EGFR mutations (Table 1, Figure 1). EGFR exon 18 intronic poylmorphism was seen in 4 cases and EGFR exon 20 showed intronic and CDS polymorphism in most cases. However, none of the cases were found to be positive for EGFR, exon 18, 20, Kras and Her-2/neu mutation. Figure 1 Figure 2
Conclusion:
These findings verify the feasibility of analysis of oncogenic drivers in cytological specimens in advanced ADC. Stained aspiration smears can be used after establishing diagnosis and checking adequacy of the specimen.
