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D.I.M. Amararathna



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    P1.01 - Poster Session with Presenters Present (ID 453)

    • Event: WCLC 2016
    • Type: Poster Presenters Present
    • Track: Epidemiology/Tobacco Control and Cessation/Prevention
    • Presentations: 1
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      P1.01-015 - Polyphenols-Rich Fruit Extracts Prevent Tobacco Specific Nitrosamine-Induced DNA Damage in Lung Epithelial Cells (ID 3734)

      14:30 - 14:30  |  Author(s): D.I.M. Amararathna

      • Abstract
      • Slides

      Background:
      Diets rich in polyphenols are well-known to reduce lung cancer risk among high-risk populations. We analyzed the efficacy of polyphenols-rich Haskap (Lonicera caerulea L.) fruit extracts in preventing tobacco specific nitrosamine (TSNA)-induced DNA damage in BEAS-2B lung epithelial cells.

      Methods:
      Monomeric polyphenols of Haskap fruits were extracted in ethanol and water, and profiled. TSNA, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and 4-[(acetoxymethyl) nitrosamino]-1-(3-pyridyl)-1-butanone (NNKOAc) were used (at sub-lethal concentrations) independently to induce the carcinogenesis process in BEAS-2B cells. Cell viability assay was confirmed that the tested concentrations of Haskap extracts were not cytotoxic to BEAS-2B cells.

      Results:
      The Haskap extracts contain diversity of polyphenols including phenolic acids and flavonoids, however, cyanidin-3-O-glucoside was the most predominant. Pre-treatment of cells with the Haskap extracts could significantly reduce the NNK- and NNKOAc-induced DNA double strand breaks, DNA fragmentation and intracellular reactive oxygen species, compared to non-treated cells. Immunocytochemistry for H2AX-phosphorylation (Serine 139, red) A. NNKOAc 100 µM, 3 h; B. Haskap ethanol extract (50 µg/mL, 3 h)+NNKOAc (100 µM, 3 h). DNA counter-staining was performed with 4,6-diamino-2-phenylindole (blue). Figure 1Figure 2





      Conclusion:
      The polyphenols-rich Haskap extracts could prevent TSNA-induced DNA damage in lung epithelial cells in vitro. Protective effects of Haskap polyphenols against DNA damage are being investigated in vivo using A/J mice.

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