Virtual Library
Start Your Search
K. Crosbie-Staunton
Author of
-
+
P3.08 - Poster Session/ Thymoma, Mesothelioma and Other Thoracic Malignancies (ID 226)
- Event: WCLC 2015
- Type: Poster
- Track: Thymoma, Mesothelioma and Other Thoracic Malignancies
- Presentations: 1
- Moderators:
- Coordinates: 9/09/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
-
+
P3.08-002 - Nanodiamonds-Conjugated Chemotherapeutic Agent Induces Cell Cycle Arrest and Apoptosis of Human Malignant Pleural Mesothelioma Cell Lines (ID 2809)
09:30 - 09:30 | Author(s): K. Crosbie-Staunton
- Abstract
Background:
The use of nanomaterials-based therapeutic systems is rapidly growing and covering a several biomedical applications such as detection, diagnosis and treatment. Recently, nanodiamonds (NDs) have been demonstrated to have great potential as a multimodal imaging/therapy platform. NDs enhance the ability of the drug to cross the cell membrane, increase intracellular drug delivery to the cancer cells, improve treatment efficacy, and decrease toxicity to normal cells or tissues. NDs are attractive for use in drug delivery because of their rich surface chemistry, advantageous size, and ability to act as transmembrane carriers. NDs have also been shown to enhance therapeutic efficacy of doxorubicin (DOX), particularly in treating murine liver and mammary tumor models, and lung metastasis of breast cancer. Yet, nothing similar has been done to translate ND-DOX systems to other cancers such as malignant pleural mesothelioma (MPM). In our study, we aimed to investigate the efficacy of ND-conjugated DOX on MPM cell lines.
Methods:
Bare, uncoated nanodiamonds were pegylated and functionalised with DOX. NDs/Dox were washed three times then re-suspended in water. Nanoparticle Tracking Analysis (NTA), FTIR and PL spectroscopy and TEM imaging were performed to characterise the functionalisation. Human MPM cell lines such as REN and NCl-H2373 and human lung carcinoma cell line (NCl-H226) were used. LP-9 cell line, which resembles normal human mesothelial cells, was used as control. All cells were cultured and exposed to NDs, NDs/DOX or DOX alone. Cell cycle, cell viability, lysosomal mass/pH changes and mitochondrial membrane potential were examined using immunofluorescent staining techniques and data were collected and analysed with high content screening tools such as Cytell and IN Cell Investigator software.
Results:
Significant alterations of the examined biological markers were detected in human MPM cell lines such (REN and NCl-H2373) and human lung carcinoma cells (H226), while a slight changes were seen in the LP-9 cells. Interestingly, the MPM cells showed greater responses to NDs/DOX versus DOX alone.
Conclusion:
Our study demonstrates that NDs loaded with DOX exert significant inhibitory activities of human MPM cell lines and at concentrations that have minimal effects on normal pleura. Thus, ND-conjugated chemotherapy represents a promising, biocompatible strategy for enhancing chemotherapy efficacy and safety.
