Author of

• 15382

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  P3.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 235)

  • Event: WCLC 2015
  • Type: Poster
  • Track: Biology, Pathology, and Molecular Testing
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/09/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)

  • 15489

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    P3.04-107 - MicroRNA Expression in Epithelial and Stromal Components of Early-Stage Non-Small Cell Lung Tumors (ID 3220)

    09:30 - 09:30  |  Author(s): W. Bshara
    • Abstract

    Background:
    MicroRNAs are ultra-short, non-coding RNAs that play important roles in the biology of lung cancer. In addition, biomarker utility of lung cancer tumor microRNAs for diagnosis, histological sub-typing, prognosis and prediction of response to therapy has been demonstrated in a large number of studies. Like all tumors, those of non-small cell lung cancer contain both cancerous epithelial and non-cancerous stromal cells. To facilitate our understanding of the role of microRNAs in lung cancer biology as well as their application as biomarkers, we examined microRNA expression in epithelial and stromal components of early-stage non-small cell lung tumors.
    
    Methods:
    Laser capture microdissection of 8 µm-thick, hematoxylin-eosin-stained sections of formalin-fixed specimens was used to separately collect epithelial and stromal components of 77 resected pathologic stage I non-small cell lung cancer tumors. Total RNA was extracted from the dissectates with the Norgen Biotek® FFPE Tissue RNA Isolation kit and quantified with Ribogreen™ assay (Invitrogen®). MiRCURY™ microarrays (Exiqon®) with locked nucleic acid hybridization probes were used to quantify microRNAs in 350 ng of each RNA isolate. For validating the microarray data, 10 microRNAs in the RNA isolates were also quantified using Taqman™ microRNA reverse transcription (RT)-PCR assays (ABI®).
    
    Results:
    Microdissection was performed for 35 adenocarcinoma, 16 bronchioloalveolar carcinoma and 26 squamous cell carcinoma tumors. Of the 1936 human mature microRNAs detectable with the microarray platform, 595 (31%) were identified as expressed and reliably quantified among the RNA samples. Microarray-based quantification of 10 microRNAs in the samples was validated by RT-PCR. Significant differences for microRNA expression between tumor epithelia and stroma, and between cancer of different histologies was noted.
    
    Conclusion:
    Our study provides information on microRNA expression in epithelial and stromal components of early-stage non-small cell lung tumors.