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K.K. Groholt
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P3.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 235)
- Event: WCLC 2015
- Type: Poster
- Track: Biology, Pathology, and Molecular Testing
- Presentations: 1
- Moderators:
- Coordinates: 9/09/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P3.04-087 - NUT Expression in Surgically Treated Small Cell, Non-Small Cell and Carcinoid Tumors of the Lung (ID 451)
09:30 - 09:30 | Author(s): K.K. Groholt
- Abstract
Background:
NUT midline carcinoma (NMT) is a rare, highly aggressive carcinoma defined by rearrangement of nuclear protein gene in testis (NUT) on chromosome 15; in most cases to bromodomain-containing protein 4 (BRD4) on chromosome 19. Although the majority of cases occur in midline structures above the diaphragm there are reports regarding cases in non-midline solid organs. There is an increased need to identify tumors with targetable mutations, and NUT-BRD4 translocations are potential goals for bromodomain and extra terminal (BET) inhibitors. The putative incidence among lung carcinomas low, but the true incidence is unknown.
Methods:
In a tissue micro array (TMA) set we investigated samples from 483 surgically resected lung tumors for the expression of the NUT protein using immunohistochemistry with monoclonal anti-NUT antibody (clone C52B1, Cell Signaling). 278 were adenocarcinomas, 140 squamous cell carcinomas, 30 large cell carcinomas, 7 small cell carcinoma, 18 carcinoid tumours and 10 carcinoma not otherwise specified. The median age were 66.3 [33.9 – 87.0], 247 were males and 236 were females. Testis and two previously confirmed NMT served as positive controls. Lymph nodes and normal lung tissue served as negative controls.
Results:
The positive controls had distinct nuclear staining without any unspecific background. The negative controls and all tumours were completely negative for the anti-NUT staining.
Conclusion:
We did not find any NUT expression in the investigated set of tumors. The golden standard for showing NUT rearrangement are fluorescence in situ hybridization (FISH), but the sensitivity and specificity for immunohistochemistry are high, 87% and 100% respectively (Haack et al. Am J Surg Pathol 2010). Although we cannot exclude a minority to be false negative, NUT translocations does not seem to be a relevant differential diagnostic issue in unselected early stage lung carcinomas.
