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A.R. Giuliano
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P3.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 235)
- Event: WCLC 2015
- Type: Poster
- Track: Biology, Pathology, and Molecular Testing
- Presentations: 1
- Moderators:
- Coordinates: 9/09/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P3.04-081 - Molecular Evidence of Viral DNA in Non-Small Cell Lung Cancer (NSCLC) and Normal Lung (ID 726)
09:30 - 09:30 | Author(s): A.R. Giuliano
- Abstract
Background:
Although 20% of human cancers are caused by infections, only suspicion exists for a microbial cause of lung cancer. This study investigated potential infectious agents in the etiology of non-small cell lung cancer (NSCLC) in both frozen tumor of the major cell types and non-neoplastic lung using several molecular methods.
Methods:
Nucleic acids were extracted from 30 frozen NSCLC [10 squamous cell (SCC), 10 adenocarcinomas (ADC), 10 bronchioloalveolar (BAC)] and 10 non-neoplastic lung tissue specimens. All specimens were screened for microbial DNA on a pan-microbial detection array containing 135,000 DNA probes and controls to detect all sequenced viral and bacterial pathogen species. Additionally, SCC specimens were evaluated by PCR for the presence of 27 subtypes of human papillomavirus (HPV) and an independent panel of 17 known or suspected oncoviruses.
Results:
RESULTS: Using the pan-microbial microarray, several species of retroviral DNA were observed in 100% of SCC, 60% of ADC, and 10% of BAC (Table 1). Among the SCC specimens, HPV DNA was found in 60%, with 30% containing one or more high-risk HPV types, but the oncovirus panel was negative. No consistent viral DNA was detected in non-neoplastic lung specimens by the pan-microbial microarray.
Table 1. Viral DNA Found in Human Lung Cancer Specimens: Pan-Microbial Array ResultsLung cancer cell type HPV (human papillomavirus, type 57) HBV (hepatitis B virus) HTLV-2 (human T- lymphotropic virus 2), a Delta-retrovirus Bovine leukemia virus (a Delta-retrovirus, similar to HTLV-1) Y53 Sarcoma virus (an Alpha-retrovirus) STLV-1, 2, or 6 (simian T-cell leukemia viruses, Delta-retroviruses) Squamous cell ca. n=10 6 (60%) 9 (90%) 7 (70%) 8 (80%) 0 8 (80%) Adenoca. n=10 1 (10%) 2 (20%) 0 0 6 (60%) 1 (10%) Bronchioloalveolar ca. n=10 0 0 0 0 1 (10%) 0 Normal lung, n=10 0 0 0 0 0 0
Conclusion:
High-risk HPV types were detected in many squamous cell lung cancers and, retroviral DNA was found in the majority of NSCLC but not in non-neoplastic lung. Of the 24 naturally-occurring animal cancers with a known etiology including lung adenocarcinoma in sheep, all are induced by retroviruses. Results from this initial discovery trial encourage further study of the viral contribution to human lung oncogenesis.
