Author of

• 15382

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  P3.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 235)

  • Event: WCLC 2015
  • Type: Poster
  • Track: Biology, Pathology, and Molecular Testing
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/09/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)

  • 15460

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    P3.04-078 - Adverse Effect of Smoking on the Intratumoral Expression of Thymidylate Synthase in Lung Cancer (ID 696)

    09:30 - 09:30  |  Author(s): S. Yamamoto
    • Abstract
    • Slides

    Background:
    Cigarette smoke is a risk factor for lung cancer. A recent study showed that lung tumors exhibited distinct epidemiological, clinical, pathological, and molecular features depending on the smoking status. Thymidylate synthase (TS) is an essential enzyme for de novo DNA synthesis. TS expression has been associated with the proliferative activity of cancer cells, and low TS expression was be associated with better outcomes for non-small cell lung cancer patients treated with TS-targeted drugs. The aim of the present study was to investigate the relationship between TS expression in lung cancer and the smoking status.
    
    Methods:
    Figure 1 The subjects were 113 patients who underwent surgical resection of lung cancer in at one of our three hospitals. Table 1 shows the patient's clinical and pathological characteristics accoding to the smoking status. We measured the intratumoral mRNA expression for TS, in manually microdissected tumor specimens using RT-PCR, and we normalized the values considering the gene for to β-actin as the reference. We analyzed the expression level of TS considering the cumulative dose of smoking in a patient’s life.
    
    
    
    Results:
    Figure 1 Table 2 shows the relationship between TS expression and the smoking dose. Among smokers, the median of smoking dose was 45 pack-years in patients with adenocarcinoma (Ad) and 50 pack-years in squamous cell carcinoma (Sq). TS expression was significantly higher in patients with Sq than in those with Ad (p = 0.0153). Among smokers with Ad, TS expression was significantly higher in patients with a smoking status of more than 45 pack-years compared to those with a smoking status of less than 45 pack-years (p = 0.0187).
    
    
    
    Conclusion:
    Our results indicate that it may be possible to predict high TS levels in patients with Ad by considering smoking dose.
    
    

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