Virtual Library
Start Your Search
G. Parks
Author of
-
+
P3.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 235)
- Event: WCLC 2015
- Type: Poster
- Track: Biology, Pathology, and Molecular Testing
- Presentations: 1
- Moderators:
- Coordinates: 9/09/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
-
+
P3.04-034 - Molecular Subtyping in Advanced Non-Small Cell Lung Cancer, a Minimally Invasive Strategy with Small Volume Fine Needle Aspirates (ID 550)
09:30 - 09:30 | Author(s): G. Parks
- Abstract
Background:
Lung cancer is the leading cause of cancer related deaths worldwide. Non-small cell lung cancer (NSCLC) accounts for the vast majority representing approximately 85-90% of cases. NSCLC is further divided into histological subtypes including adenocarcinoma (AC) and squamous cell (SCC). Significant treatment implications exist according to histological classification which can be difficult due to scarcity of tissue or poor cellular differentiation. Patients often present with advanced disease thus making small volume, minimally invasive biopsy techniques ideal. Small volume biopsies, however, present an inherent challenge in obtaining sufficient amounts of high quality cancer cell-specific genomic material for testing and diagnostics. Here we tested the RNA yields from several minimally invasive techniques. We utilized two separate platforms to test a previously determined adeno-squam signature. We hypothesized that RNA yields would be sufficient for molecular histologic classification from a single needle biopsy. We sought to compare the yields of small volume biopsy techniques to RNA extracted from larger volume fresh frozen surgical specimens.
Methods:
Forty-eight individuals with suspected lung cancer underwent diagnostic biopsy with the standard approaches utilizing trans-thoracic needle biopsy (n=22) and transbronchial needle aspiration (n=26). RNA was extracted from a single pass specimen after the diagnostic biopsies were obtained (multiple passes). The total mass (ug), RNA integrity number (RIN) and % mass equal to or above 300 base pairs were recorded for all specimens. Statistical t-test analysis was performed on subgroups with focus on yield and quality. RNA from both FNA specimens as well as fresh frozen surgical specimens (n=44) obtained from a tumor bank at our institution were analyzed using Nanostring technology with the previously identified specific gene panel (A/S signature) obtained on the Quantigene platform.
Results:
Histological classification of FNA samples included adenocarcinoma (n=24), squamous cell (n=16) and NSC-NOS (n=8). Mean values for all FNA specimens included total mass of 1.58 ug, RIN of 4.0, and 85.4% mass equal to or above 300 base pairs. Fresh frozen surgical specimens including adenocarcinoma (n=21) and squamous cell (n=23) underwent successful RNA isolation with mean total mass of 45.2 ug, RIN of 6.1, and 68.8% mass equal to or above 300 base pairs. Differential histological gene expression occurred for both FNA and fresh frozen surgical specimens on the Nanostring platform.
Conclusion:
RNA isolation from NSCLC related small volume tissue biopsies is possible among several minimally invasive FNA techniques. Small volume tissue biopsy RNA yields are a sufficient means for molecular analysis and histological subtyping. We have successfully validated differential histological expression on two separate platforms from both single pass FNA techniques and frozen tumor samples. Given the increasing prevalence of such techniques and evolution of molecular analysis this may prove to be a powerful research and diagnostic tool.
