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L. García-Alanís
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P3.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 235)
- Event: WCLC 2015
- Type: Poster
- Track: Biology, Pathology, and Molecular Testing
- Presentations: 1
- Moderators:
- Coordinates: 9/09/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P3.04-028 - Defining the Molecular Profile of Non-Small Cell Lung Carcinoma in a Comprehensive Cancer Center in Mexico City (ID 868)
09:30 - 09:30 | Author(s): L. García-Alanís
- Abstract
Background:
Molecular characterization of lung cancer is of paramount importance. Although genetic drivers such as EGFR,KRAS and ALK mutation are routine, they represent 30-40% of all mutations identified. The relative frequency vary according to the population studied and scarce data exist on Mexican population. The aim of the study is to describe the clinicopathologic characteristics and molecular profile of the population of NSCLC patients studied in a recent molecular pathology laboratory.
Methods:
Cases diagnosed with NSCLC from January 2012 to March 2015 seen at the department of surgical and molecular pathology of TheAmerican-BritishCowdrayMedical Center in Mexico City were retrieved. Medical records were reviewed for data on clinicopathologic characteristics (age, sex, biopsy site, histological parameters, and mutational status of EGFR, KRAS and ALK. DNA extraction was done using QIAampDNAFFPE Tissue Kit(Qiagen). EGFR and KRAS determination was performed using scorpion-ARMS technique(Therascreen/Qiagen) in Rotor-GeneQThermalcycler(Qiagen). ALK rearrangement was determined using ALK-LSI probes(Abbott Molecular), and evaluated with OlympusBX53 fluorescence microscope. All the procedures were carried out according to manufacturer instructions.
Results:
90 cases were retrieved, 77(85.6%) adenocarcinoma, 6(6.7%) squamous cell carcinoma, 3(3.3%)large cell carcinoma and 4(4.4%) mixed cells types (2 adenosquamous carcinoma, 1 adenocarcinoma with neuroendocrine component, and 1 sarcomatoid carcinoma). Histologic subclassification showed predominant acinar in 56%, solid 20%, lepidic 15%, and 9% micropapillary pattern. Lung biopsies were the tissue specimen in 58 cases(64.4), metastatic site in 28(31.1)(lymph node 9, bone 8, pleura 3, skin 2, soft tissue 2, mediastinal tumor 1, ovary 1, parotid 1 and CNS 1), and non-specified 4(4.5%). Demographic variables and mutational status of EGFR/KRAS/ALK are shown in table 1. Figure 1 Figure 2
Conclusion:
We corroborate in our population the higher frequency of EGFR mutation in female patients, with a percentage between Caucasian and Asian populations. KRAS is the most frequent mutation and mutually exclusive with EGFR and ALK. Triple negative cases represent half of NSCLC.
