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A. Sandal
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P2.08 - Poster Session/ Thymoma, Mesothelioma and Other Thoracic Malignancies (ID 225)
- Event: WCLC 2015
- Type: Poster
- Track: Thymoma, Mesothelioma and Other Thoracic Malignancies
- Presentations: 1
- Moderators:
- Coordinates: 9/08/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P2.08-012 - Differentiation of Mesothelioma from Lung Cancer and Healthy Individuals Using Human Serum by ATR-FTIR Spectroscopy Coupled with Chemometrics (ID 2553)
09:30 - 09:30 | Author(s): A. Sandal
- Abstract
Background:
Malignant pleural mesothelioma (MPM) is a rare, aggressive cancer that progresses in the thin layer of pleura. The inhalation of microscopic asbestos fibers primarily the main reason of the disease. Early symptoms of pleural mesothelioma often are confused with other respiratory ailments. Therefore, MPM can only be diagnosed in the advanced stage. Consequently, it is important to develop a new approach with high specificity and sensitivity for the differential diagnosis of MPM. Fourier Transform Infrared (FTIR) spectroscopy is a novel and non-invasive method that has potential to diagnose cancer with high specificity and sensitivity. Hence, we used ATR-FTIR spectroscopy coupled with chemometric methods to differentiate MPM from healthy individuals and lung cancer patients.
Methods:
FTIR spectra of the samples collected from patients diagnosed with malignant pleural mesothelioma (MPM), lung cancer (LC), and healthy control (C) were recorded in the 4000-650 cm[-1]spectral region. Recording the spectra and analysis of the spectral data were obtained with Perkin Elmer Spectrum One Program. Both unsupervised Principal Component Analysis (PCA) (The Unscrambler X 10.3, CAMO Software) and Hierarchical Cluster Analysis (HCA) (OPUS 5.5, BRUKER) were applied for differentiation MPM from other groups.
Results:
Cluster analysis of the samples demonstrated that MPM and lung cancer successfully differentiated from healthy controls at whole spectral region (4000-650 cm[-1]). Moreover, successful clusters of these three groups were obtained in the fingerprint (1800-650 cm[-1]) and 1500-800 cm[-1 ]regions. In addition, some special bands such as DNA band at 832 cm[-1 ]gave very successful differentiation by PCA from serum samples.
Conclusion:
These findings indicate that FTIR spectroscopic analysis of serum coupled with chemometric analyses enabled differential diagnosis of mesothelioma from lung cancer patients and healthy individuals. *This work was supported by the Scientific and Technical Research Council of Turkey (TUBITAK), SBAG-113S294 Research Fund.
