Author of

• 14490

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  P2.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 234)

  • Event: WCLC 2015
  • Type: Poster
  • Track: Biology, Pathology, and Molecular Testing
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/08/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)

  • 14570

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    P2.04-080 - Novel Biomarkers for Non Small Cell Lung Cancer (NSCLC) (ID 1117)

    09:30 - 09:30  |  Author(s): L. Mirandola
    • Abstract

    Background:
    Abstract Cancer testis antigens (CTA) are a class of tumor associated antigens, showing a restricted expression in cancer, strong immunogenicity, and weak expression in normal tissues. Sp17/AKAP4/PTTG1 have been previously investigated, showing promising results as a target. Our aim was to investigate the expression of Sp17/AKAP4/PTTG1 in lung cancer patients.
    
    Methods:
    We analyzed 2 lung cancer cell lines, one normal bronchus cell line, a panel of normal tissues and patients cells by RT-PCR, flow-cytometry, immunocytochemistry (ICC), and immunofluorescence (IF). CTA immunogenicity was investigated by measuring circulating specific antibodies in the sera of lung cancer patients.
    
    Results:
    ELISA analyses show the presence of circulating CTA-specific antibodies in the sera of lung cancer patients, indicating the immunogenicity of Sp17, AKAP-4 and PTTG-1. Sp17/AKAP4/PTTG1 were detected only in the cells of lung cancer patients.
    
    Conclusion:
    We showed that CTA, Sp17, AKAP-4 and PTTG-1 can be detected in both sera and tissue of patients with NSCLC.