Author of

• 14490

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  P2.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 234)

  • Event: WCLC 2015
  • Type: Poster
  • Track: Biology, Pathology, and Molecular Testing
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/08/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)

  • 14568

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    P2.04-078 - Functional Characterization of NK Cells in Non-Small Cell Lung Cancer (ID 2975)

    09:30 - 09:30  |  Author(s): Á. Gómez-Gallegos
    • Abstract
    • Slides

    Background:
    Lung cancer is the leading cause of cancer death worldwide and most of the patients are diagnosed with advanced disease. Lung cancer is the leading cause of cancer death worldwide. Natural killer (NK) cells are important effector cells in control of infected, malignant, and tumor cells. The aim of this study was to investigate the activation state and cytotoxic potential of NK peripheral cells in patients with Non-Small Cell Lung Cancer (NSCLC).
    
    Methods:
    We investigated the relationship between NK cells apoptosis and Fas expression. NK cell apoptosis, Fas and Fas-L, NKG2D, CD69, KIR, CD244, CD122 and CD161 receptors were evaluated with multiparametric flow cytometry. We further evaluated the cytotoxic activity of NK cells and IFN-gamma expression. For this purpose, we simultaneously analyzed the loss of intracellular perforin and the surface expression of CD107a/b as well as the intracellular IFN-gamma expression with multiparametric flow cytometry.
    
    Results:
    Our results showed that Fas-positive NK cells in lung cancer patients were higher than healthy controls (P<0.001). These results also showed that up-regulation of Fas expression is related to increased apoptosis of circulating NK cells. Regarding the cytotoxic capacity, our results showed that upon PMA stimulation, the expression of surface CD107a/b and loss of intracellular perforin of NK cells from patients with NSCLC were not correlated indicating an impaired functional cytotoxic activity. Interestingly, we also found that, IFN-gamma (P<0.005) and NKG2D expression were also impaired significantly (P<0.001).
    
    Conclusion:
    The results from this study suggest a possible NK cells anergy state. Our description will help to provide a mechanistic insight into tumor immune escape via negative regulation of NK cell innate function; however, the underlying mechanisms remained to be addressed.
    
    

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