Author of

• 14490

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  P2.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 234)

  • Event: WCLC 2015
  • Type: Poster
  • Track: Biology, Pathology, and Molecular Testing
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/08/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)

  • 14546

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    P2.04-056 - Analysis of EGFRvIII, PIK3CA, and DDR2 Mutations in Chinese Lung Squamous Cell Carcinoma Patients (ID 300)

    09:30 - 09:30  |  Author(s): J. Tan
    • Abstract
    • Slides

    Background:
    As the role of targeted therapy of lung adenocarcinoma affirming, people pay more attention to targeted therapy of squamous cell lung cancer . EGFRvIII , PIK3CA and DDR2 genes become research hotspots as targeted therapy of potential squamous cell lung cancer. However there are few reports on the state of these genes in Chinese patients. Therefore, this study intends to conduct PIK3CA, DDR2 and EGFRvⅢ genetic testing on the surgical specimens from Chinese lung squamous cell carcinoma patients, in order to understand the gene status of Chinese lung squamous cell carcinoma patients and its relationship with clinical factors.
    
    Methods:
    This study collected 100 surgical tissue samples of lung squamouscarcinoma patients from Xiangya Hospital of Central South University. All patients had not received preoperative radiotherapy and chemotherapy. EGFRvIII gene was detected by Immunofluorescence，PIK3CA and DDR2 was detected by direct sequencing. X[2 ]test was applied to analyze the relationship of each gene mutation, expression and clinical characteristics (age, gender, smoking status, tumor stage, tumor differentiation), and two-sided test P <0.05 is considered statistically significant.
    
    Results:
    Patient Enrollment A total of 100 cases met eligibility for this study. Clinicopathologic characteristics of 100 patients are shown in Table 1.

    Table 1 Detailed Clinicopathologic Characteristics of 100 Lung Squamous Cell Carcinomas

    Variable	No. (%)
    Total patients	100
    Age
    ＜60y	59(59)
    ≥60y	41(41)
    Sex
    Male	92(92)
    Female	8(8)
    Smoking
    Smoker	82(82)
    Never Smoker	18(18)
    TNM stage
    Stage I	20(20)
    Stage II	23(23)
    Stage IIIa	57(57)
    Stage IIIb	0(0)
    Stage IV	0(0)
    Differentiation
    Poor	26(26)
    Moderate	65(65)
    Well	9(9)

    EGFRvIII expression and mutational spectrum of PIK3CA, DDR2 In the 100 cases of lung squamous cell carcinoma specimens, 15 cases were positive of EGFRvIII gene staining (15%); 5 cases of PIK3CA mutation (5%), of which all occurred on 9th exon E545K; among all tested specimens, no DDR2 mutation was found. Correlation between clinicopathologic characteristics and status of EGFRvIII expression, and PIK3CA mutantion The EGFRvIII gene expression and PIK3CA gene mutation in the patients’ age, gender, smoking, TNM stage and tumor differentiation had no statistically significant difference.
    
    Conclusion:
    This study found out that the PIK3CA mutation rate in Chinese lung squamous cell carcinoma patients was higher than in Westerners.
    
    

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