Virtual Library
Start Your Search
P. Chandrani
Author of
-
+
P1.12 - Poster Session/ Community Practice (ID 232)
- Event: WCLC 2015
- Type: Poster
- Track: Community Practice
- Presentations: 1
- Moderators:
- Coordinates: 9/07/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
-
+
P1.12-010 - Bench to Bedside Detection of Actionable Genotypes by SNaPshot for Lung Cancer Panel (ID 314)
09:30 - 09:30 | Author(s): P. Chandrani
- Abstract
Background:
Conventional therapeutic solutions in NSCLC are not effective to treat the disease. Despite of all developments in understanding of the disease, mortality of lung cancer patients remains high. Recent developments of personalized therapy have given promising results in terms of improved survival of NSCLC patients. Thus, we were keen to develop a cost effective and sensitive diagnostic lung cancer panel assay for targetable mutation detection by using SNaPShot PCR technique on FFPE samples.
Methods:
Method: Multiplexed (SNaPShot) PCR was optimized to amplify hotspot regions from 9 targetable genes followed by single base extension reaction using fragment analysis on ABI 3500 Sequencer. Gene Mapper software was used for analysis
Results:
The successfully developed mutation profiling assay was divided into 3 multiplexed reactions, covering 23 actionable genotypes of EGFR, KRAS, BRAF, PIK3CA, Her2, AKT1, NRAS, MEK1 and PTEN genes. The assay was standardized and validated on 20 blood samples, 10 cell lines and 20 FFPE samples expressing good sensitivity and specificity for wild type and mutant genotypes.
Conclusion:
This In house developed SNaPShot PCR technology is robust, economical, specific and sensitive to detect actionable mutations in FFPE Adeno as well as in Squamous Carcinoma samples. Because these variants have differing genetic, biological, and clinical properties, including response to treatment, this Bench to Bedside research will lead us to correct classification of lung cancer cases and will assure that lung cancer patients receive optimum management.
