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Z. Altun
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P1.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 233)
- Event: WCLC 2015
- Type: Poster
- Track: Biology, Pathology, and Molecular Testing
- Presentations: 1
- Moderators:
- Coordinates: 9/07/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P1.04-115 - Chemoterapy and Targeted Therapy Sensitivity Testing in Malignant Pleural Effusions (ID 1643)
09:30 - 09:30 | Author(s): Z. Altun
- Abstract
Background:
Clinical management of malignant pleural effusions (MPE) is a major problem in oncology with short survival. MPE is caused by various types of malignancies, especially lung, breast carcinomas, lymphomas besides ovarian carcinoma, malignant melanoma. Intrapleural chemotherapy might be a helpful treatment strategy in MPE cases. Intrapleural chemotherapy also enters systemic circulation and affects the primary tumor as well. The aim of this study is to evaluate ex vivo chemoterapy and targeted therapy sensitivity in MPE cases to project which drug might be effective.
Methods:
Effusion fluids from patients with MPE were fresh obtained. After centrifugation, the pellet was diluted in PBS and cell isolation was performed by Ficoll gradient to separate cells from erytrocytes. Cells were incubated in HITES supplemented complete RPMI medium at 37C with 5%CO2. After primary cell culture was obtained, cells were incubated to 96 well plates and agents ( Bevacuzimab, Cetuximab, Rituximab, Bortozemib, Gemsitabin, Vinblastin, Bleomycin, Docetaxel, 5 Florourasil, Cisplatin, Cyclophosphamide, Doxorubicine) in different dose ranges for 24 hours. WST-1 was performed to check cell viability.
Results:
The primary tumors of nine cases in this study with MPEs are breast carcinomas, lung adenocarcinoma, small cell carcinoma and mantle cell lymphoma. Resistance were observed in most drugs. Breast carcinoma cells and lung adenocarcinoma cells were sensitive to cisplatin and/or vinsblastin. Small cell carcinoma cells were sensitive to docetaxel and/ or bleomycin. Sensitivity was not observed to targeted therapy agents at single dose during 24 hours incubation.
Conclusion:
Our results indicate that ex vivo cancer cell culture and testing cell death results of various chemotherapoetic and new targeted drugs might help managing highly agressive disease of patients with MPE. Intrapleural chemotherapy application that is found sensitive by ex vivo tests might help patients.
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P3.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 235)
- Event: WCLC 2015
- Type: Poster
- Track: Biology, Pathology, and Molecular Testing
- Presentations: 1
- Moderators:
- Coordinates: 9/09/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P3.04-100 - Chaperonin (HSP60) and Annexin 2 Are Candidate Biomarkers for Early Diagnosis of Non Small Cell Lung Carcinoma (ID 1645)
09:30 - 09:30 | Author(s): Z. Altun
- Abstract
Background:
Lung cancer is responsible of 12.4% and 17.6% of all newly diagnosed cancer cases and mortality due to cancer respectively and 5-year survival rate despite all improved treatment options is 15%. This survival rate reaches 66% in the Stage 1 and surgically treated patients. Early diagnosis which could not be definitely and commonly achieved yet is extremely critical in obtaining high survival rate in this disease. For this reason; proteomic differences were evaluated using MALDI TOF/TOF mass spectrometry in the subgroups of lung adenocarcinoma and squamous cell carcinoma.
Methods:
Fresh tissue samples of 36 malignant cases involving 83.3% (n=30) male and 16.7% (n=6) female patients were distributed into two groups as early and end stage lung cancer and each group were composed of subgroups including 18 squamous cell carcinoma (9 early stage cases, 9 end stage cases) and 18 adenocarcinoma cases (9 early stage cases, 9 end stage cases). Of the malignant cases, 41.7%, 7.3%, 44.4% and 5.6% were at Stage 1, Stage 2, Stage 3 and Stage 4, respectively. 50.0% (n=18) and 50.0% (n=18) were classified as early and final stage cases, respectively. The fresh tissues obtained from the tumoral and matched normal sites after surgical intervention. The differences in protein expression levels were determined by comparing proteomic changes in the tumoral tissues with normal tissues in each patient. The results obtained following two dimensional gel electrophoresis and MALDI TOF/TOF mass spectrometry were detected with respect to differences in protein densities of the subgroups identified by Decodon two dimensional gel analysis system.
Results:
In the subgroups of advanced stage adenocarcinoma; tumoral tissue revealed differences in expression of 2 proteins compared with normal parenchymal tissue. Of those; difference in protein expression in HSP60 (heat shock protein 60) was found statistically significant (p=0.0001). On the other side, subgroups of early and advanced stage squamous cell carcinoma revealed differences in expression of 20 particular proteins. Of those, increased protein expression level of only annexin-2 protein was found statistically significant (p=0.002). No significant difference was detected in early and advanced stage protein expressions of the tumoral tissues in the subgroups of adenocarcinoma and squamous cell carcinoma.
Conclusion:
n the light of these results; we conclude that with respect to early diagnosis of lung cancer that HSP60 and annexin-2 proteins are the important biomarkers in the subgroups of adenocarcinoma and squamous cell carcinoma. We also consider that these two proteins are molecules which may provide critical contribution in evaluation of prognosis, metastatic potential, response to treatment and in establishment of differential diagnosis between adenocarcinoma and squamous cell carcinoma.
